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桃(Prunus persica)中2个MADS box基因功能的初步鉴定和遗传作图
  • 期刊名称:科学通报? 2008 53(3) 537-543
  • 时间:0
  • 分类:S662.1[农业科学—果树学;农业科学—园艺学] Q943[生物学—植物学]
  • 作者机构:[1]College of Life Science, Capital Normal University, Beijing 100037, China, [2]Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
  • 相关基金:Supported by the National Natural Science Foundation of China (Grant No. 30500395), the National High Technology Research and Development Program of China (Grant Nos. 2006AAIOZI30 and 2006AA100108-3-7) and Beijing Natural Science Foundation (Grant No. 5073046)
  • 相关项目:桃花发育相关MADS box 基因的功能研究及基因定位
中文摘要:

以前,无性器官的基因相当或相同事物 PpMADS4 和 FRUITFULL 基因相当或相同事物 PpMADS6 从桃树(Prunus pers 集成通信适配器) 被孤立,并且两基因在发展被显示出到快车花并且水果。到进他们的函数的获得卓见,二基因组成地在 Arabidopsis thaliana 被表示,他们工厂生长和花的机关开发上的效果在这个工作被学习。转基因的工厂都显示了开花期的早 flowering 和变换到花的分裂组织。然而,二基因在 A 在花的机关结构上有不同效果。thaliana。在表示 PpMADS4 上的转基因的工厂显示了花的机关的回家耳的变换,并且特别地,花被截去被禁止。在表示显示出的早 flowering,心皮的生产更高的数字,花瓣,和比非转基因的工厂的雄蕊,和豆荚粉碎的 PpMADS6 上的工厂被阻止;显著地,转基因的工厂从一朵单个花产出超过一 siliques。一个 SSR 分子的标记为 PpMADS4 被开发,并且它然后被分配进 Prunus sp 的 G5 连接组。PpMADS4 和 PpMADS6 基因在一样的区域位于 G5 连接组。我们的结果证明这二 MADS 的潜在的应用程序为庄稼和水果树改进关基因。

英文摘要:

Previously an AGAMOUS gene homologue PpMADS4 and a FRUITFULL gene homologue PpMADS6 were isolated from peach (Prunus persica), and both genes were shown to express in the developing floral and fruits. To gain insight into their function, the two genes were constitutively expressed in Arabidopsis thaliana and their effects on plant growth and floral organ development were studied in this work. The transgenic plants all displayed early flowering and conversion of inflorescence to floral meristem. However, the two genes had different effects on the floral organ structures in A. thaliana. The transgenic plants overexpressing PpMADS4 displayed homeotic conversion of floral organs, and particularly the perianth abscission was inhibited. The plants overexpressing PpMADS6 showed early flowering, produced higher number of carpels, petals, and stamens than nontransgenic plants, and pod shatter was prevented; significantly, the transgenic plants yielded more than one siliques from a single flower. A SSR molecular marker was developed for PpMADS4, and it was then assigned into the G5 linkage group of Prunus sp. Both PpMADS4 and PpMADS6 genes were located at the same region in the G5 linkage group. Our results showed the potential application of these two MADS box genes for crop and fruit tree improvement.

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