中文摘要：

目的：研究奥沙利铂对TRAIL诱导胃癌细胞凋亡的影响,探讨死亡受体5（DR5）在TRAIL诱导细胞凋亡中的作用。方法：采用MTT法测定细胞活力、采用流式细胞仪检测细胞凋亡,采用Western blot检测DR5蛋白表达。结果：100ng/ml的TRAIL导致轻度的增殖抑制,诱导不超过5%的细胞凋亡;TRAIL（100ng/ml）联合奥沙利铂（23.44μg/ml）引起明显的细胞增殖抑制和细胞凋亡（P〈0.05）,TRAIL没有明显改变死亡受体5（DR5）的表达,而23.44μg/ml奥沙利铂作用胃癌细胞24小时后,明显上调了DR5的表达。结论：奥沙利铂通过上调DR5的表达增强TRAIL诱导的胃癌细胞凋亡。

英文摘要：

Objective：To investigate the effect of Oxaliplatin on TRAIL-induced apoptosis in human gastric cancer cells,and the role of death receptor 5（DR5） in TRAIL-induced cell apoptosis.Methods：Cell proliferation was measured using MTT assay.Cell apoptosis was determined by flow cytometry.Expression of proteins was analyzed by Western blot.Results：100 ng/ml TRAIL resulted in a slight reduction in cell viability in MGC803 cells.Treatment with TRAIL alone caused no more than 5% cell apoptosis.Treatment with TRAIL（100 ng/ml） and Oxaliplatin（23.44 μg/ml） for 24 h resulted in a significant reduction in cell viability and a dramatic increase in cell apoptosis（P0.05）.TRAIL alone did not significantly change the expression of death receptor 5（DR5）,while 23.44μg/ml Oxaliplatin significantly upregulated DR5 expression at 24 h.Conclusion：Oxaliplatin enhanced TRAIL-induced gastric cancer cell apoptosis through upregulating DR5 expression.