中文摘要：

探讨肝细胞粘附分子（hepatocyte cell adhesion molecule,hepa CAM）对膀胱癌细胞BIU-87增殖活力的影响以及其调节机制。将BIU-87细胞分为对照组、空载组、hepa CAM组、LY294002组及hepa CAM与LY294002联用组,噻唑蓝法（methyl thiazolyl tetrazolium,MTT）检测细胞的增殖抑制率;Real-time PCR检测增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）的表达;Western blot法检测p-FOXO1的表达;同时用LY294002处理BIU-87细胞后Western Blot检测p-FOXO1、PCNA的表达。结果显示,单独运用hepa CAM过表达腺病毒或LY294002均能够明显抑制膀胱癌BIU-87细胞的增殖活力,hepa CAM过表达腺病毒与LY294002联用更加显著的增强了单独作用的抑制效果（p〈0.05）;Western Blot显示,hepa CAM基因的过表达使p-FOXO1的表达量显著降低（p=0.001）;hepa CAM过表达腺病毒与LY294002联用后,与hepa CAM或LY294002分别作用相比,p-FOXO1（p=0.014,p=0.047）和PCNA（p=0.002,p=0.004）的表达量降低明显;Realtime PCR结果显示,LY294002处理BIU-87细胞后,PCNA的表达明显减低（p=0.003）,加入hepa CAM过表达腺病毒后更为显著地增强LY294002对PCNA的抑制作用（p=0.001）。本课题得出结论,hepa CAM基因过表达后通过下调p-FOXO1从而抑制膀胱癌BIU-87细胞的增殖活力,此作用与PCNA的表达量下调有关。

英文摘要：

This study aimed to explore the effect of hepa CAM（hepatocyte cell adhesion molecule） on cell proliferation in bladder cancer cell BIU-87 and the potential mechanism. We divided BIU-87 cells into control group,no-load group, hepa CAM group, LY294002 group, and hepa CAM＋LY294002 group, then we detected the inhibition rate of BIU-87 cell by MTT（methyl thiazolyl tetrazolium） assay, identified the gene expression of PCNA（proliferating cell nuclear antigen） by Real-time PCR, determine the protein expression of p-FOXO1 by Western blot, and measured the expression of p-FOXO1 and PCNA after treatment with LY294002 by Western Blot. The results showed that proliferation of bladder cancer BIU-87 cell was remarkably decreased by the adenovirus of over-expressed hepa CAM or LY294002 treatment alone, and combined treatment with adenovirus of hepa CAM and LY294002 decreased the cell viability more significantly（p〈0.05）. Western blot assay showed that over-expression of hepa CAM reduced the protein expression of p-FOXO1（p=0.001） markedly, but compared with the respective of hepa CAM or LY294002, the expression decreased significantly（p-FOXO1： p=0.014, p=0.047; PCNA： p=0.002, p=0.004） after the use of combined injection by ad-hepa CAM and LY294002. Real-time PCR showed that the expression of PCNA decreased significantly（p=0.003） after the treatment of BIU-87 cell by LY294002, while the inhibition effect of LY294002 on PCNA got stronger after over-expressed hepa CAM virus was added（p=0.001）. Our study showed that the over-expressed hepa CAM inhibited the proliferation of BIU-87 cell through downregulating p-FOXO1,which may be related to the decreased expression of PCNA.