2008—2009年,应用FACE研究平台,采用烟农19、扬麦16、嘉兴002和扬辐麦2号4个小麦品种,以O3自然浓度为对照,研究了大气O3浓度增高50%对不同基因型小麦剑叶显微结构、叶绿素含量和粒重的影响.结果表明:在开花期,大气O3浓度增高对小麦产生轻微伤害,剑叶的叶肉细胞和叶绿体结构开始遭到破坏,基粒片层断裂并且开始松散;花后21d,与对照的叶片微结构差异显著增大,剑叶内膜系统基本解体,叶绿体基本解散,基粒片层消失,加速了剑叶的衰老进程,剑叶的叶绿素含量、净光合速率和成熟期粒重均低于对照.小麦剑叶微结构和光合功能对大气O3浓度增高的响应存在明显的基因型差异,嘉兴002对O3胁迫的耐受性较好,而扬辐麦2号对O胁迫的反应较敏感.
By using FACE (Free-Air Controlled Environment)-ozone system, a field plot experiment was conducted in 2008-2009 to study the effects of elevated ozone ( O3 ) concentration on the flag leaf microscopic structure, chlorophyll content, and grain weight of wheat. Two treatments were installed, i. e. , ambient 03 and 150% of ambient 03, and four winter varieties, i. e. , Yannong 19, Yangmai 16, Jiaxin 002, and Yangfumai 2, were taken as the test materials. At anthesis, elevated O3 concentration had a slight damage to the flag leaf microscopic structure. The mesophyll cell and chloroplast structure began destroying, and the grana lamellae started breaking and loosing. Twenty-one days after anthesis, the differences in the leaf microscopic structure between the two treatments became significant. Under elevated O3, the flag leaf senescence was accelerated, with the endomembrane system disintegrated, grana lamella disappeared, and corpus adiposum inside chloroplast broken down, resulting in a significant decrease of the chlorophyll content, photosynthesis rate, and grain weight at maturing stage. Significant difference was observed among the test varieties in their responses to elevated O3. Jiaxin 002 was tolerant, while Yangfumai 2 was sensitive to the ozone stress.