中文摘要：

目的：检测上海地区膀胱癌患者尿沉淀细胞中3个肿瘤相关基因启动子CpG岛的甲基化谱式异常频率，继而评估其应用前景。方法：采用甲基化特异性PCR方法分析尿沉淀细胞DNA中DAPK1、bcl2和hTERT3个基因的启动子CpG岛甲基化状态，并通过RT-PCR方法评估DAPK1基因在膀胱癌细胞系中的表达状态。结果：对膀胱癌细胞系中DAPK1基因的启动子CpG岛甲基化状态及其表达（mRNA水平上）所做的分析，确立了高甲基化状态与表达静息化之间的相关性。对46例临床确诊的膀胱癌患者和84例非膀胱癌对照（包括前46例术后的36例）的尿沉淀细胞中DNA甲基化的分析发现，仅bcl～2基因的高甲基化见之于28．3％（13／46例）的膀胱癌患者，而84例的对照中均为去甲基化状态。结论：在美国膀胱癌患者尿沉淀细胞中频发DNA高甲基化的靶点在上海地区膀胱癌患者人群中频率很低，因此寻找在后者中频发DNA高甲基化的新靶点实属必要。

英文摘要：

Objective：To determine the abnormal methylation profile of promoter CpG island of the three oncogenes, DAPK1 ,bcl 2, and hTERT, in the urine sediments of Shanghai bladder cancer patients and evaluate the application foreground of this method. Methods： Methylation specific polymerase chain reaction method （MSP） was used to analyze the methylation state of promoter CpG islands of the three genes in a bladder cancer cohort （46 cases） and the non bladder cancer control （84 cases）. Reversed transcriptase polymerase chain reaction method was used to quantify the expression of DAPK1 gene in each of the three bladder cancer cell lines. Results： Hypermethyla tion state of promoter CpG islands of DAPK1 genes was related with the silenced state of gene transcription at mRNA level. DNA methy lation analysis showed that the three genes were in unmethylated status in the control cohort and only promoter CpG island of bcl 9 gene was hypermethylated in 13 cases out of 46 （28. 3%） bladder cancer patients. Conclusion： The hypermethylated bcl-2 gene in the urine sediments of American bladder cancer patients only have the limited value in Shanghai bladder cancer patients. It is necessary to search for the new targets of DNA hypermethylation in Shanghai bladder cancer patients.