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中文摘要：
在pH1．98时，色氨酸和氯金酸发生氧化还原反应生成金纳米粒子。用扫描电镜、紫外-可见吸收光谱和荧光光谱对所生成的金纳米粒子进行了表征，并通过在普通荧光光度计上测定所生成的金纳米粒子的光散射信号，建立了定量检测色氨酸含量的方法。方法的线性范围为0．21～60．0μmol／L；检出限为0．02μmol／L。甘氨酸等10余种无还原性的氨基酸对体系不产生干扰。将所建立的方法用于复方氨基酸注射液中色胺酸的测定，相对误差小于5．7％；回收率在96．3％～104．6％之间。
英文摘要：
A redox reaction occurs between tryptophan and HAuCl4 in a medium of pH 1.98, producing Au nanoparticles that were characterized with UV-Vis and fluorescence spectra and scanning electron microscopic images. With a common spectrofluorometer to measure the light scattering signals of the formed Au nanoparticles, a new method for the determination of tryptophan has been developed in linear range of 0. 21 - 60.0μmol/L with a limit of detection of 0.02μmol/L. It was found that most amino acids have no interfering effects in the determination, and therefore the present method could be applied for the detection of tryptophane in compound amino acids injection. The recovery was 96.3% -104.6%, and the relative error was lower than 5.7%.
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