中文摘要：

探究Rho激酶抑制剂Y-27632对间充质干细胞（mesenchymal stemcells，MSCs）C3H10T1／2增殖和成脂分化的影响．实验分为对照组、成脂诱导组和Y-27632处理组（Y-27632＋成脂诱导）．利用MTT检测细胞增殖情况，油红O染色，异丙醇萃取法检测细胞成脂分化情况，半定量RT—PCR检测过氧化物酶体增殖物激活受体γ（peroxisome proliferator activiated receptor1，PPARγ）和CCAAT增强子结合蛋白α（CCAATenhancerbindingproteinα，C／EBPα）基因表达．结果表明，Y-27632能够显著抑制C3H10T1／2细胞的增殖（P〈0．05），并呈一定的浓度依赖性；高浓度Y-27632对C3H10T1／2细胞成脂分化具有显著抑制作用（P〈0．05）；半定量RT-PCR结果显示，成脂诱导处理组PPARγ，和C／EBPα表达量在第3d、5d和7d显著低于成脂诱导组（P〈0．05）．综上所述，Y-27632能够抑制C3H10T1／2细胞增殖与成脂分化．

英文摘要：

To investigate the effect of Rho kinases inhibitor Y-27632 on the proliferation and adipogenic differentiation of mesenchymal stem cells, the C3HIOT1/2 cells were cultured in DMEM medium with 10% fetal bovine serum at 37 ℃ in 5% CO2 incubator and divided into the control, adipogenic and Y- 27632 groups. The cell proliferation was detected by MTT; the adipogenic differentiation was characterized by oil red O staining. The C3H10T1/2 cell differentiation into adipocytes was also examined by the gene expression of PPARy and C/EBPa by RT-PCR. The results showed that Y-27632 inhibited the proliferation of C3H10T1/2 cell in a dose-dependent manner （P 〈 0.05 ）. High concentrations of Y-27632 inhibited adipogenic differentiation of C3H10T1/2 cell （P 〈0.05）. The RT- PCR assays showed that the PPAR7 and C/EBPe~ expression were inhibited by Y-27632 at day 3, 5 and 7 during differentiation as compared to the eontrol （P 〈 0.05）. In summary, Y-27632 is able to inhibit the proliferation and adipogenic differentiation of C3H10T1/2 cell.