中文摘要：

目的探讨供体抗原特异性CD4＋CD25＋调节性T细胞（Treg），对同种异体复合组织移植（CTA）受体大鼠细胞免疫耐受功能的影响。方法采用免疫磁珠法分选CD4＋CD25＋Treg，将数量1×10^6的CD4＋CD25＋Treg输注入CTA受体的大鼠，术后30d采取外周血，尼龙毛柱分离T、B细胞，检测在IgM抗体刺激下的增殖放射强度的液体闪烁测定（CPM）值及血清IgG和IgA水平，判断CD4＋CD25＋Treg对T、B细胞功能的影响及CTA的存活情况，并进行统计学分析。结果分选的CIM＋CD25＋Treg纯度为95．6％。T、B细胞CPM值：正常对照组分别为2436±358、2418±348，Treg局部干预组为2273±136、2252±127，Treg全身干预组分别为2338±228、2315±218，Treg未干预组分别为3749±245、3720±224；Treg未干预组与其三组比较差异均有统计学意义（P〈0．01）。血清IgG、IgA水平：Treg局部和全身干预组分别为（12．56±1．30）g/L、（2．38±0．21）g/L和（13．48±1．23）g/L、（2．86±0．24）g/L，与正常对照组（12．35±1．28）g/L、（2．36±0．12）g/L比较差异无统计学意义（P〉0．05），三组与Treg未干预组（16．58±1．12）g/L、（3．75±0．37）g/L比较差异有统计学意义（P〈0．01）。Treg局部和全身干预组CTA存活时间分别为（97±13）和（63±10）d，显著长于Treg未干预组的（22±8）d（P〈0．01）。结论供体抗原特异性C1M＋CD25＋Treg对T、B细胞功能有明显的抑制作用，能诱导CTA免疫耐受，延长其存活时间，且局部应用效果优于全身。

英文摘要：

Objective To approach the effect of the donor antigenic specificity CD4＋ CD25 ＋ regulatory T cell （Treg）on cellular immune tolerance function in rat composite tissue allotransplantation （CTA）. Methods Use the method of immunomagnetic heads to separate CD4＋ CD25 ＋ Treg, （ 1 × 10^6） CD4 ＋CD25＋ Treg was transfused to rat CTA model. Collected peripheral blood 30 days after operation, and used nylon wool column to separate B cell and T cell. With the stimulation of lgM, detected B cell proliferation and the level of IgG and IgA in serum. Observed the effect of CD4＋ CD25＋ Treg on B cell and T cell function and the survial of allotransplants, and analyzed the data by statistics. Results The purity of separated CD4 ＋ CD25 ＋ Treg was 95.6%. The CPM of T cell of normal control group, topical intervention group, systemic intervention group and non-intervention group were （2436 ± 358）, （2273 ± 136）, （2338 ± 228） and （3749±245）. The CPM of B cells of normal control group, topical intervention group, systemic intervention group and non-interventiom group were （ 2418 ±348 ） , （ 2252 ± 127 ） , （ 2315 ±218 ） and （3720±224）, there was a significant difference in these groups （P 〈0. 01 ）. The serum level of IgG and IgA of topical intervention group and systemic intervention group were （ 12. 56 ± 1.30 ） , （ 2. 38± 0. 21 ）, （13.48±1.23） and （2.86±0.24） g/L, and of normal control group was （12.35±1.28）, （2.36± 0. 12） g/L, had no significant difference （P 〉0. 05）. But Treg of non-intervention group was （16. 58± 1. 12） , （3.75 ±0. 37） g/L, there was a significant difference in the non-intervention group and the three above groups （P 〈 0.01 ）. The survival time of CTA in intervention of local and systemic groups were （97 ±13） and （63 ± 10） d, which were significant longer than the non-intervention group [ （22 ± 8） d, P 〈 0. 011. Conclusions Donor antigen specific CD4＋ CD25＋ Treg has signi