中文摘要：

目的：观察针刺心包经穴对大鼠心肌缺血／再灌注损伤过程中心肌细胞Na^＋-K^＋-ATP酶和Ca^2＋Mg^2＋-ATP酶活性的影响，探讨其心肌保护作用的机制。方法：50只大鼠随机分为5组。假手术组只穿线不结扎冠状动脉，其余各组制备缺血／再灌注动物模型，其中3个针刺穴位组分别于电针大鼠内关穴、郄门穴或支沟穴20min后，结扎冠状动脉左前降支40min，心电图监测，再电针相应穴位20min，恢复灌流。假手术组于穿线后100min、模型组和各针刺穴位组于再灌注60min摘取心脏，取心室肌制备心肌细胞膜．定磷法观察Na^＋-K^＋-ATP酶和Ca^2＋Mg^2＋-ATP酶活性的变化。结果；针刺心包经穴（内关、郄门）组Na^＋-K^＋-ATP酶和Ca^2＋-Mg^2＋ LATP酶活性均明显升高．与模型组比较差异均非常显著（P均〈0．01）。结论：针刺手厥阴心包经穴可提高Na^＋-K^＋-ATP酶和Ca^2＋-Mg^2-ATP酶活性，减轻心肌细胞钙超载程度，有效地保护心肌。

英文摘要：

Objective： To study the effects of acupuncture at the acupoints of hand-Jueyin （手厥阴心包经穴） on the activities of Na^＋- K^＋- ATPase and Ca^2＋- Mg^2＋ ATPase during the process of ischemia/ reperfusion （I/R） iniury in rats and investigate its mechanism. Methods： Fifty rats were randomly divided into five groups with 10 rats in each group. In the sham-operated group, a thread was passed through the artery, but I/R model was not established. The other groups were replicated I/R model, in which the three acupuncture groups were given acupuncture at the acupoints of Neiguan （内关穴）, Ximen （郄门穴 ） or Zhigou （支沟穴） respectively for 20 minutes, followed by ligation of the left anterior descending branch of the coronary artery for 40 minutes with electrocardiogram monitoring, afterwards, the acupoints were acupunetured again for 20 minutes, and then reperfusion was performed for 60 minutes. The myocardial tissue samples （below the ligation site） of the left cardiac ventricle were processed into cell membranes, and the two kinds of ATPase were examined by the method of fixing phosphorus. Results ： In the acupuncture groups, the activities of Na^＋- K^＋- ATPase and Ca^2＋- Mg^2＋- ATPase were increased obviously compared with that of the model group （all P〈0.01）. Conclusion： Acupuncture at the aeupoints of hand-Jueyin can obviously improve the activities of Na^＋ K^＋ ATPase and Ca^2＋ Mg^2＋-ATPase. Thus, it may reduce the degree of intracellular calcium overload, and protect myocardial performance.