中文摘要：

目的：探讨丹参注射液（SMI）在链霉素（SM）肾毒性损伤过程中的拮抗作用及其机制。方法：40只豚鼠随机分为4组：正常对照组、SM组、SMI＋SM组、SMI组。应用光镜、电镜、免疫组化、原位末端标记法及图像分析等技术，观察应用SM后肾脏病理改变、诱生型一氧化氮合酶（iNOS）、碱性成纤维细胞生长因子（bFGF）的表达、凋亡发生以及尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）的活性。结果：用药10d后，光镜及电镜下可见SM组肾脏病理改变严重，SMI＋SM组损伤较轻，同时凋亡细胞数明显少于SM组。免疫组化结果表明，SM组iNOS的表达明显高于SMI＋SM组，而bFGF的表达低于SMI＋SM组。另外，SMI＋SM组NAG活性明显低于SM组（P〈0．01）。结论：丹参注射液能降低SM肾毒性损伤时iNOS的过强表达，减少NAG的活性，增强bFGF的表达，减少凋亡发生，从而对链霉素所致肾损伤具有拮抗作用。

英文摘要：

AIM： To investigate the antagonistic and protective mechanism of salviae miltiorrhizae injection （SMI） on kidney toxicity damaged by streptomycin （SM）. METHODS： 40 guined pigs were divided randomly into 4 groups： the control group, SM group, SMI ＋ SM group and SMI group. Light microscope, transmission electron microscope （TEM）, immunohistochemical staining, TdT mediated biotin dUTP nick -end cabelling （TUNEL） techniques and image quantitative analysis technique were used to observe the change of kidney after administration of SM and SMI. The expressions of iNOS and bFGF, apoptosis, and the activity of N - acetyl - D - glucosaminidase（NAG） were also observed. RESULTS： 10 d after administration of SM, the serious pathological change in SM group was observed under the light microscope and transmission electron microscope. Slight damage was found in SMI ＋ SM group, and the number of apoptosis cells was fewer than that in SM group. The result of immunohistochemical implied that the iNOS expression in SM group was higher than that in SMI ＋ SM group, while the bFGF expression was lower. The activity of NAG in SMI ＋ SM group was lower than that in SM group （P 〈 0.01 ）. CONCLUSION ： Salvia mihiorrhiza injection inhibits the increased expression of iNOS during kidney toxicity damaged by SM, reduces the activity of NAG, increases the expression of bFGF, and decreases the apoptosis, hence has the antagonism to the kidney toxicity damaged by SM.