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中文摘要：
利用5-甲基四氢叶酸自身产生荧光的特性，建立高效液相荧光检测法来测定柑橘中5一甲基四氢叶酸的含量。使用ODS～C18反相色谱分离柱，流动相为6％乙腈，94％0．05mol／L磷酸二氢钾溶液（pH3．0）作为流动相，流速1．0mL／min，柱温23％，进样量为20斗L，激发波长为290nm，发射波长为360nm。结果表明，5-甲基四氢叶酸在O．003μg／mL-0．1μg／mL有良好的线性关系，相关系数为0．9999，检出限为0．01ng／mL，样品测定的精密度为0．99％，样品加标平均回收率为96．6％。建立的高效液相荧光检测法具有良好的精密度和回收率，适合用于柑橘中5一甲基四氢叶酸的测定。
英文摘要：
A sensitive and specific high performance liquid chromatography-fluorescence detection （RP-HPLC-FLD）） method was developed for the quantitative determination of 5-methvltetrahvdrofolic acid （5-MTHF） in citrus, using the acid＇s fluorescence-emitting characteristics. The determination was performed on a ODS C18 reverse-phase column en- closed in a heater maintained at 23℃, the mobile phase was a mixture of 94% 0.05mol/L aqueous potassium phosphate buffer （pH 3.0 ） containing 6% acetonitrile, and the flow rate was 1 mL/min. The loop size was 20μL, the optimized exci- tation wavelength was 290nm and the emission wavelength was 360nm. The results showed that the detection limits were 0.01ng/mL, the linear range was 0.003μg/mL - 0.1μg/mL, the correlation coefficient was 0.9999, the recovery was 96.6%, and the precision of the method was 0.99%. It was concluded that this method, being simple, fast and accurate, had high sensitivity, recovery and good repeatability and was suitable for the determination of 5-MTHF in citrus.
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