中文摘要：

对橙色红曲菌As3.4384及其诱变菌株大米固态发酵物中桔霉素和色素的HPLC分离进行了优化,采用Symmetry C18（250mm×4.6mmi.d.,5μm）色谱柱,流动相为乙腈-水（磷酸调pH3.0,77∶23,V/V）,分别采用紫外检测器（UV=254nm）、荧光检测器（λex=331nm,λem=500nm）及两者串联对桔霉素和色素进行了监测。通过ESI-MS对色素进行了鉴定,并测定了经橙色红曲菌As3.4384及其诱变菌株于30℃发酵14d后的大米、糯米、小黄米、燕麦米和红米中桔霉素含量。结果表明：ESI-MS鉴定的两种色素分别为红曲菌素和红曲黄素,橙色红曲菌As3.4384发酵后的大米、糯米、小黄米、燕麦米和红米中桔霉素含量分别为151.4、100.5、5.06、80.5和150.5mg/kg,而诱变菌株发酵后的大米、糯米、小黄米、燕麦米和红米中桔霉素含量分别为0.218、3.86、0.223、2.45和4.05mg/kg。

英文摘要：

Citrinin and pigments were produced in solid-state fermentation rice by Monascus aurantiaaeus （As3. 4384） and its mutant strains（ CSM-11 ） at 30℃ for 14 d. The reversed phase high performance liquid chromatographic conditions have been optimized for the separation of citrinin and pigments. The chromatographic separations were carried on a symmetry C18 （250 mm×4.6 mm I. D. , 5 μm） column by using acetonitrile-H2O （77：23, V/V, pH 3.0） as mobile phases, Citrinin and pigment were monitored by ultraviolet detector （UVD） （A 254 nm）, fluorophotomeric detector （FD）（λex = 331 nm, λem = 500 nm） and both connected in series. Meanwhile, pigments were identified by ESI-MS. The contents of citrinin of fermentation rice, glutinous rice, yellow millet, A. satival rice and red rice by As3. 4384 and CSM-11, respectively, were determined. The results show that the pigments were monascine and ankaflavine. The contents of citrinin of fermentation rice, glutinous rice, yellow millet, A. satival rice and red rice by As3. 4384 were 151.4, 100. 5, 5.06, 80.5 and 150.5 mg/kg, respectively, and by CSM-11 were 0. 218, 3.86, 0. 223, 2.45 and 4.05 mg/kg, respectively.