中文摘要：

以具有不同开花习性的9份芸薹种作物为试材,根据开花相关基因BrFLC2序列设计引物进行PCR扩增,发现9份材料均获得约1 400 bp的片段。通过克隆测序分析发现9份材料存在片段大小差异,因此开发了与此相关的BrFLC2的InDel标记。通过对来自8个不同栽培种群的146份自然群体材料的基因型检测与开花时间的相关性分析发现：开花时间早晚与BrFLC2的InDel的基因型显著相关（r=0.412,P〈0.01）。Del基因型材料开花时间（平均71 d）明显比In基因型（平均109 d）早。研究证明该标记与开花时间表型显著相关,可以用来进行分子标记辅助选择育种。

英文摘要：

Nine Brassica campestiris accessions with a wide range of flowering time variation were used for screening of sequence variation of BrFLC2.Using gene specific primer pair,a fragment about of 1 400 bp was generated from all accessions.Sequencing of fragments revealed there were fragment length differences among the nine accessions,and an insertion/deletion（InDel）marker was developed for BrFLC2 based on the sequence variation.We screened the flowering time and the genotype of 146 accessions from eight different cultivar groups and found that：The flowering time was significantly correlated to the genotype of In/Del genotype of BrFLC2（r = 0.412,P 0.01）.The flowering time of Del genotype accessions（average at 71 days）were much earlier than the In genotype accessions（average at 109 days）.These results indicated that InDel marker was significantly correlated to the flowering time and can be easily used for marker-assisted selection（MAS）in breeding.