东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

磷胁迫对烤烟高亲和磷转运蛋白基因表达及磷素吸收利用的影响

ISSN号：1000-4025
期刊名称：《西北植物学报》
时间：0
分类：Q945.79[生物学—植物学] Q789[生物学—分子生物学]
作者机构：[1]河南农业大学烟草学院/国家烟草栽培生理生化研究基地,郑州450002, [2]河南省烟草公司许昌市公司,河南许昌461000
相关基金：中国烟草总公司特色优质烟叶开发重大专项浓香型项目（Ts-01-2011005）

作者：王艳丽[1], 王京[1], 刘国顺[1], 丁松爽[1], 李建华[2]

关键词：烤烟, 磷胁迫, 高亲和磷转运蛋白基因, 磷素吸收利用, flue-cured tobacco, deficient phosphorus stress, high-affinity phosphate transporter gene, phosphorus absorption and utilization

中文摘要：

为探明缺磷胁迫对烤烟磷吸收的影响机理,以烤烟品种‘豫烟10号’为材料,采用盆栽试验设置不施磷（-P）和正常供磷（＋P）2个处理,分析了烟草生育后期根系和叶片中高亲和磷转运蛋白基因NtPht1;1、NtPht1;2（简称PT1、PT2）的表达差异性及其表达量与烟草磷含量、磷积累量的关系。结果表明：（1）随生育期的推进,烤烟根系和叶片的PT1基因相对表达量、PT2基因相对表达量、干物质重和磷素积累量逐渐增加,烟叶的磷含量逐渐降低,根系磷含量无明显的变化规律。（2）磷胁迫促使PT1、PT2基因的表达量上调,其中叶片PT1基因的相对表达量高于根系,叶片PT2基因的相对表达量显著低于根系。（3）磷胁迫限制了烤烟对干物质和磷素的积累,在移栽后90d,-P处理烤烟根系和叶片的干物质重、磷素积累量不到＋P处理的一半,处理间差异极显著。研究认为,在缺磷环境下,烟草高亲和磷转运蛋白基因PT1和PT2表达量的增加,是由磷胁迫下烟草体内磷积累量降低所引起的系统性调控。

英文摘要：

In order to clarify the mechanism of deficient phosphorus stress on phosphorus absorption of flue-cured tobacco,taking ‘Yuyan No.10’ as the material,we designed a pot experiment with two treatments,which were no phosphate fertilizer application and recommended amount of phosphate fertilizer.The expression differences of high-affinity phosphate transporter genes NtPht1;1（PT1） and NtPht1;2（PT2） between two treatments and the correlation of genes expression and phosphorus accumulation in leaves and roots at later growth stages were analyzed.The results showed that：（1）with the advancement of the growth stages,the relative expression of PT1 and PT2 genes,the dry matter weight and the phosphorus accumulation in leaves and roots of flue-cured tobacco increased gradually and the content of phosphorus in leaves decreased.Whereas,there was no significant pattern in roots.（2）The relative expression of PT1 and PT2 genes were up-regulated under deficient phosphorus stress.The relative expression of PT1 gene in leaves was higher than that in roots,while PT2 gene was the opposite,comparatively.（3）The accumulation of dry matter and phosphorus in flue-cured tobacco were limited under deficient phosphorus stress.At 90 days after transplanting,the dry matter weight and phosphorus accumulation in tobacco roots and leaves with no phosphate fertilizer application were less than half the values of the phosphorus application treatment.There were extremely significant differences among treatments at 0.01 probability levels.This might imply that the up-regulated expression of PT1 and PT2 genes under deficient phosphorus stress was due to the decrease of phosphorus accumulation in tobacco tissues rather than the low available phosphorus soil.

同期刊论文项目