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中文摘要：
建立了光度法测定辣根过氧化物酶（HRP）活性的季胺-过氧化氢-HRP新体系，探讨了反应机理．该方法基于含KI的pH4．5PBS介质中，HRP催化H2O2氧化季胺[二（4-二甲氨基苯基）甲烷]的显色反应在462nm处的吸光度．吸光度与HRP活性呈线性关系．该可溶性的季胺比目前临床常用显色剂3,3’，5，5’-四甲基联苯胺更稳定，克服了后者的缺点．在选定的实验条件下，测定HRP的线性范围为2．0×10^-9～2．5×10^-7g／mL，检出限为3×10^-10mg／mL．应用于HRP标记马抗人甲胎蛋白免疫标记物的测定，结果满意．该方法操作简便，灵敏度高，在临床上有较好的应用前景．
英文摘要：
A new spectrophotometric system of tetrabase [bis（4-dimethylaminophenyl）methane]-H2O2- horseradish peroxidase （HRP） was developed for the detection of HRP activity, and the reaction mechanism was discussed. The detection was based on the HRP catalyzing coloring reaction of tetrabase in pH 4.5 PBS containing KI, which behaved maximum absorption at 462 nm. The absorbance was proportional to the activity of HRP. This new tetrabase reagent is soluble and more stable＇ than 3,3＇,5,5＇-tetramethyl benzidine （TMB）, a usually used coloring reagent for ELISA in clinic, and could overcome the shortcomings of TMB. Under optimal conditions, the linear range for HRP detection was 2 × 10^-9-2.5 × 10^-7 g/mL with the detection limit of 3 × 10^-10 g/mL. This system could be used for the determination of HRP labeled horse anti-human a-fetoprotein with satisfactory results. The new system is simple, rapid and sensitive, and has good application prospect in clinic.
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