中文摘要：

目的探讨血管内皮生长因子（vascular endothelial growth factor，VEGF）在体外对人急性白血病细胞株HL-607调亡的影响。方法采用形态学观察、单细胞凝胶电泳、流式细胞仪等方法对分别经过VEGF和（或）VP16处理过的急性白血病细胞株HL-60细胞凋亡进行检测。结果各种不同浓度VEGF（2μg／L、20μg／L、100μg／L）任孵育18h后可明显抑制急性白血病细胞株HL-60的凋亡，并且可对抗VP16诱导的细胞凋亡效应。结论VEGF可以抑制人急性白血病细胞的凋亡，可能是白血病的发病机制之。

英文摘要：

Objective To investigate the effect of vascular endothelial growth factor （VEGF） on apoptosis of human acute myeloid leukemia HL-60 cell line in vitro. Methods The morphology of HL-60 cell and its apoptosis were detected by single-cell gel electrophoresis （SCGE） and flow cytometry （FCM）. Resuits After treatment with VEGF（2μg/L、20μg/L、100μg/L） for 18 h, the apoptosis of HL-60 cells was inhibited in the way of dose dependence, and VEGF could also inhibite the apoptosis induced by Etoposide （VP-16） exposure. Conclusions The inhibitory effect of VEGF on apoptosis may play an important role in the pathogenesis of human acute myeloid leukemia.