中文摘要：

肠道菌群与宿主肥胖密切相关,伪无菌小鼠动物模型是研究肠道菌群与肥胖及其他相关疾病关系的有利＂工具＂。20只8周龄C57BL/6J雄性小鼠给予抗生素混合物饮水,剔除大部分肠道微生物模拟无菌动物。随机分成两组：对照组和高脂组,分别饲喂常规纯化饲料与高脂纯化饲料8周;同时以20只SPF级小鼠作对照。常规方法测定小鼠体重、血清中血糖、血脂指标和胰岛素水平,并用软件测量脂肪细胞大小。结果表明,与高脂组SPF小鼠相比,高脂组伪无菌小鼠的体重、脂肪垫与肾周脂重量显著低于高脂组SPF小鼠;胰岛素水平和脂肪细胞大小也显著低于SPF小鼠。高脂组伪无菌小鼠出现低体重低脂肪的表型,与无菌小鼠动物模型极为相似。因此采用抗生素处理建立伪无菌小鼠动物模型可用来预示无菌小鼠试验的结果,是研究肠道菌群与某些疾病的理想动物模型。

英文摘要：

Gut microbiotas are closely related with the obesity of their host. Pseudo germ-free mice are a useful animal model for studying the relationship of gut microbiota with obesity and other related diseases. Twenty C57BL/6J eight-week-old male mice were administered with antibiotic cocktails in drinking water to remove the gut microbiota to establish the pseudo germ-free mouse model. These mice were randomly divided into two groups： control（CONV） group and high fat（HF） group, which were fed with a normal diet and a high-fat diet for eight weeks, respectively. Meanwhile, 20 specific-pathogen-free（SPF） mice were used as controls. Body weight, serum glucose levels, serum lipid levels, and insulin concentrations of the mice were measured by normal methods, and the areas of fat cells were calculated using software. The results revealed that body weight, fat pad, and perirenal fat weight of the HF pseudo germ-free mouse group were significantly lower than those of the HF SPF mouse group（p〈0.001）, and the insulin level and areas of fat cells in the HF pseudo germ-free mouse group were also significantly lower than those of SPF mice（p〈0.01）. Pseudo germ-free mice in the HF diet group showed low bodyweight and a low-fat phenotype, which were extremely similar to those of germ-free mice. As a result, the pseudo germ-free mouse model, established using antibiotic cocktails, can be used to predict modulation results in germ-free mice and is an ideal animal model for exploring the relationship between gut microbiota and diseases.