中文摘要：

Endostatin ，骨胶原 18a 的C终端碎片，由禁止 angiogenesis.However ，为大规模生产要求的纯化过程和这些过程的伴随的费用在 vivo 禁止确定的肿瘤和转移的生长和在临床的测试的低有效性，建议其他的交货方法可能为 endostatin.In 的有效治疗学的使用被要求现在的学习，我们有人的 endostatin 基因表达向量的 transfected Chinesehamster 卵巢( CHO )房间并且在生物学上活跃的 endostatin 的 alginate-poly-L-lysine microcapsules.The 版本包含了 CHOcells 用鸡 chorioallantoic 膜试金被证实。当注入了老鼠的腹的洞时，包含的 endostatin-expressingCHO 细胞能在一个下的 B16 肿瘤模型禁止主要肿瘤的生长。这些结果在癌症治疗拓宽 themicroencapsulated 细胞 endostatin 交货系统的临床的申请。

英文摘要：

Endostatin, a C-terminal fragment of collagen 18a, inhibits the growth of established tumors and metastases in vivo by inhibiting angiogenesis. However, the purification procedures required for largescale production and the attendant cost of these processes, together with the low effectiveness in clinical tests, suggest that altemative delivery methods might be required for efficient therapeutic use of endostatin. In the present study, we transfected Chinese hamster ovary （CHO） cells with a human endostatin gene expression vector and encapsulated the CHO cells in alginate-poly-L-lysine microcapsules. The release of biologically active endostatin was confirmed using the chicken chorioallantoic membrane assay. The encapsulated endostatin-expressing CHO cells can inhibit the growth of primary tumors in a subcutaneous B 16 tumor model when injected into the abdominal cavity of mouse. These results widen the clinical application of the microencapsulated cell endostatin delivery system in cancer treatment.