中文摘要：

目的 建立对二球悬铃木花粉过敏的特应性体质者的HLA—DRB1等位基因的PCR-SSP检测方法，探讨江苏汉族人二球悬铃木花粉变应原特应性体质者与HLA—DRB1等位基因的相关性。方法 用酚-氯仿法提取全血DNA，合成8时等位基因特异性引物，用PER—SSP方法检测20例江苏汉族二球悬铃木花粉过敏体质者和36例健康人HLA—DRB1＊0401，＊0402，＊0403，＊0404，＊0405，＊0406，＊0407，＊0408等位基因。结果经优化实验争件，建立了HLA—DRB1的8个等位基因的PCR—SSP检测方法，获得了患者组和健康人组上述HLA-DRB1等位基因的分布资料。二球悬铃木花粉变应原特应性体质者DRB1＊0405和＊0406基因频率高于健康人对照组而DRB1％0402基因频率低于健康人对照组。其他5个等位基因频率二组间无显著性差异。结论 HLA-DRB1＊0406和＊0405可能是对二球悬铃木花粉变应原过敏的Ⅰ型变态反应特应性体质者的可疑候选易患等位基因，而DRB1＊0402基因可能是与之相关的具有抵抗作用的等位基因。

英文摘要：

Objective To develop a PCR-SSP method for detection of HLA-DRB1 alleles in the patients who were hypersensitive to Platanus Aeerifolia pollen allergen ,and to probe into the association between the atopic subjects to Platanus Acerifolia pollen allergen and HI,A-DRB1 alleles. Methods DNA in whole blood was extracted by phenol-chlorofor,n method. Eight pairs of specific primers for alleles were synthesized,and HLA-DRB1 ＊ 0401, ＊ 0402, ＊ 0403, ＊ 0404, ＊ 0405, ＊ 0406, ＊ 0407, ＊ 0408 alleles in 20 atopic patients and 36 heahhy individuals of Jiangsu Province with Han nationality were detected by PCR-SSP（ polymerase chain reaction-sequence specific primer）. Results By optimizing the experimental conditions PCR-SSP methods for detection of the 8 alleles were established and the distributing data of above-mentioned HLA DRB1 were obtained. The frequency of HLA DRB1 ＊ 0405 and ＊ 0406 in the patients group was higher than that of in healthy controls group,while the frequency of HLA DRB1 ＊ 0402 in the patients group was lower than that in controis, No significant deference for the other 5 alleles was found between the 2 groups. Conclusion HLA-DRB1 ＊ 0406和 ＊ 0405 seems to he the likely suspected candidate alleles responsible for susceptibility to Platanus Acerifolia pollen allergen in the atopic patients, while DRB1 ＊ 0402 might be contribute to the related resistance to the allergen.