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Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum

ISSN号：0253-505X
期刊名称：《海洋学报：英文版》
时间：0
分类：S63[农业科学—蔬菜学;农业科学—园艺学] S188[农业科学—农业基础科学]
作者机构：[1]Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China, [2]Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
相关基金：The National Basic Research Program of China （973 Program） under contract No.2010CB428706; the National Natural Science Foundation of China for Creative Research Groups under contract No.41121064; the National High Technology Research and Development Program of China under contract No.2008AA09Z107.

作者： LIU Yun[1,2], SONG Xiuxian[1], HAN Xiaofian[1], YU Zhiming[1]

关键词：硝酸盐含量, 转录水平, 中肋骨条藻, 蛋白基因, 营养条件, 转运, 逆转录聚合酶链反应, 硝酸盐浓度, high-affinity nitrate transporter gene, phosphate starvation, nitrate concentrations, real-time PCR, Skeletonema costatum

中文摘要：

为了作为氮地位，抄本的层次与磷酸盐饥饿联系了的在抄本的变化和不同硝酸盐集中的指示物验证高亲密关系的硝酸盐 transporter 基因(Nrt2 ) 的可行性，在 Skeletonema costatum 的批文化用即时量的反向抄写的 PCR (QRT-PCR ) 技术被学习。结果证明与 P 饱满的状况相比， P 饥饿能显然减少 Nrt2 抄本层次。Nrt2 抄本层次在 40 桴浲 ? 絅? 下面与硝酸盐集中有重要否定线性关联 ? 金尸尬整瑸 ? 噭素屻摣瑯素瑜硥筴挠 ? 幽 ? 紱屻摣瑯素瑜硥筴传 ? 幽 ? 紱 ????? 金 ? 噭氠摥琠? 档湡吗？？

英文摘要：

To verify the feasibility of high-affinity nitrate transporter gene （Nrt2） as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR （QRT-PCR） technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 pmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built： y = -3.305x ＋ 98.95, R2 = 0.988, where x represents nitrate concentrations （〈40 btmol/L） and y represents the Nrt2 transcript levels.