中文摘要：

目的观察雷公藤内酯醇（TP）对内毒素（LPS）激活小鼠腹腔巨噬细胞（MФ）分泌促炎递质一氧化氮（NO）和白细胞介素-6（IL-6）的影响。方法分离纯化MФ，用LPS激活，与不同浓度TP进行培养，以Griess试剂检测培养上清液中的NO含量；以ELISA法检测培养上清液中的IL-6的浓度。结果TP在浓度0．01-10．00μg·mL^-1范围内，时间4～24h范围内，对MФ产生NO具有显著抑制作用（P〈0．01），且呈剂量和时间依赖关系；TP在浓度0．001～10．000μg·mL^-1范围内，时间12h，对M中产生IL-6具有显著抑制作用（P〈0．01），呈剂量依赖关系。结论TP可以抑制被LPS激活的MФ活性，具有高效低毒的抗炎作用。

英文摘要：

Objective To study the effect of triptolide （TP） on NO and IL-6 of peritoneal elicited macrophage （MФ） activated by lipopolysaccharide（LPS） in mice. Methods The peritoneal elicited macrophage were separated, purified and activated by LPS in mice,then cultured in vitro with a series concentration of TP. The activity of NO and level of IL-6 in cellular supernatants were determined by Griess reagent and ELISA, respectively. Results The activity of NO in MФ was significantly inhibited （P 〈 0.01） by TP（0.01 - 10.00μg·mL^-1） during 4 -24 h in a time and dosage dependent manner;The level of IL-6 was obviously decreased（P 〈 0.01 ） by TP（0. 001 - 10. 000 μg·mL^-1 ） in 12 h in a dose-dependent way. Conclusion TP could inhibit the NO activity and IL-6 level in macrophage activated by LPS, and it has strong anti-inflammatory effects with low toxicity.