中文摘要：

目的：克隆和构建带大鼠结缔组织生长因子（CTGF）基因真核表达载体pcDNA3．1（＋）／CTGF，并观察其对血管外膜成纤维细胞（AF）表型的影响。方法：以AF总RNA为模板，进行逆转录-聚合酶链反应（RT—PCR），获得CTGF的cDNA，克隆入pcDNA3．1（＋）载体，酶切和测序鉴定重组子。将构建好的pcDNA3．1（＋）／CT-GF用脂质体法转入AF细胞。Western blotting观察CTGF的表达。同时观察转染pcDNA3．1（＋）／CTGF对细胞表型的影响。结果：成功构建大鼠CTGF基因的真核表达载体pcDNA3．1（＋）／CTGF．并证明其能在真核细胞内表达。转染pcDNA3．1（＋）／CTGF可以诱导AF表型转化。结论：重组结缔组织生长因子可诱导大鼠血管外膜成纤维细胞表型转化。

英文摘要：

AIM： To construct pcDNA3.1 （ ＋ ）/connective tissue growth factor （CTGF） eukaryotic expression plasmid and to investigate its role in the promotion of phenotypic transition in adventitia fibroblasts （AF）. METHODS： The expression vector pcDNA3. 1 （ ＋ ）/CTGF was constructed by routine molecular biological method. The expression vector pcDNA3.1 （ ＋ ）/CTGF was confirmed by restriction enzyme digestion and sequencing method. The expression vector pcDNA3.1 （ ＋ ）/CTGF was transfected into AF and the exogenous expression was observed. The expression of the α - SM actin was examined by Western blotting. RESULTS： The eukaryotic expression vector of CTGF was successfully constructed, which was transfected into AF, the expressed CTGF promoted phenotype transition in AF. CONCLUSION： The pcDNA3.1 （ ＋ ）/CTGF plasmid was constructed and transfected into AF, the expressed CTGF promoted phenotype transition in AF.