中文摘要：

以新疆乌恰县4个样地新疆沙冬青Ammopiptanthus nanus根围3个土层土壤为研究对象，利用Nested-PCR-DGGE技术，结合DGGE图谱分析、DNA克隆测序及系统发育分析，研究新疆沙冬青AM真菌菌群结构与遗传多样性特征，为新疆沙冬青根围功能菌群研究奠定基础。结果表明，不同样地和土层，AM真菌具有不同DGGE指纹图谱特征，AM真菌丰度、优势度及其多样性指数均有差异，其中上阿图什30-40cm土层AM真菌丰度和多样性指数最高，分别为20和3．48。DGGE条带测序和系统发育分析显示，全部序列归为2属14种，球囊霉属Glomus是其优势属，其中Uncultured Glomus（AB698612）和Uncultured Glomus（AB698616）普遍存在于4样地3个土层，Glomussp．（FN429104）和Uncultured Glomus（KC797120）是上阿图什30-40cm土层特有种，Glomussp．（EU332717），Rhizophagus intraradices（FR750206）和Uncultured Glomus（AB698616）是新疆沙冬青根围优势种。G．indicum和Rh．intraradices在形态学鉴定中未出现，说明分子生物学技术对真菌多样性研究起着补充和完善的作用。

英文摘要：

Ammopiptonthus nonus is an excellent sand-fixation and endangered plant that can not only well stand up to desert environment, but also maintain the balance and stability of desert ecosystem. To elucidate the community composition and genetic diversity of AM fungi in the rhizosphere of A, nanus by PCR-DGGE and DNA cloning and sequencing, samples from four different sites in Wuqia, Xinjiang were collected in June 2012. Soil samples were taken at depth intervals of 10-20cm, 20-30cm and 30-40cm. The results showed that the fingerprint characteristics of DGGE, richness, dominance and diversity index of AM fungi varied with sampling sites and soil depth. Richness and diversity index of AM fungi were the highest at 30-40cm soil layer n Shang Artux, reaching 20 and 3.48 respectively. 20 AM fungal sequences coincide with 14 species in two genera, Glomus and Rhizophagus. G. indicum and Rh. intraradices were not recognized by morphological definition