中文摘要：

【目的】比较分析葱蝇Deliaantiqua非滞育与夏滞育蛹的蛋白表达差异，为进一步揭示昆虫滞育的分子调控机理和昆虫防治提供理论基础。【方法】以非滞育和夏滞育的蛹为材料，提取总蛋白；进行双向凝胶电泳和凝胶图像分析，对并差异蛋白质进行MALDI-TOFMs质谱鉴定，获得该点的质量指纹图谱；利用MASCOT软件在NCBI和SWISSPORT蛋白质数据库中进行搜索鉴定。【结果】非滞育和夏滞育蛹的蛋白表达存在显著差异。通过质谱鉴定和生物信息学分析，13个差异表达的蛋白质分别为胶原蛋白、纺锤丝组装非正常蛋白6（SAS6）、5，10．亚甲基四氢叶酸合成酶（MTHFS）、Bnb蛋白（banglesandbeads）及其他功能未知蛋白。【结论】葱蝇在夏滞育时期，蛹体内的某些蛋白被上调或下调表达。本研究所鉴定的蛋白中，部分可能是参与滞育相关的蛋白质网络中的成员，它们可能在抗高温、染色体分离、叶酸代谢等生理过程中发挥重要作用。

英文摘要：

[ Aim ] Comparative analysis was performed to investigate differentially expressed proteins between non- and summer-diapausing pupae of the onion fly, Delia antiqua, and to provide a theoretical basis for further exploring the molecular regulation mechanisms of insect diapause and pest control management. [ Methods ] Total proteins were extracted from non- and summer-diapausing pupae, separated by two-dimensional gel electrophoresis, and then analyzed by image analysis. Peptide mass fingerprinting of differential proteins were analyzed by MALDI-TOF MS and identified through database searching in NCBI and SWISS PORT using MASCOT. [ Results ] Proteins were differentially expressed in non-and summer-diapausing pupae. Thirteen differentially expressed proteins were identified by mass spectrometry and bioinformatic analysis, including collagen, SAS6 （spindle assembling abnormal protein 6）, MTHFS （5, 10-methenyhetrahydrofolate synthetase）, Bnb （bangles and beads） and proteins with unknown functions. [ Conclusion ] The expressions of some proteins are up- or down-regulated in summer- diapausing pupae. Proteins identified in this study may be members of diapause-related protein regulatory networks, and they potentially play important roles in high temperature resistance, chromosome separation, folate metabolism and other physiological processes.