利用开顶箱熏蒸法,研究了高浓度O3(≈80nmol·mol^-1)和高浓度CO2(≈700μmol·mol^-1)及其复合处理对蒙古栎叶片活性氧代谢的影响.结果表明:高浓度O3显著增加了蒙古栎叶片超氧阴离子(O2^·-)产生速率、过氧化氢(H2O2)和丙二醛(MDA)含量和电解质外渗率(P〈0.05),显著降低了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性和抗坏血酸(AsA)含量(P〈0.05).高浓度CO2对蒙古栎叶片活性氧代谢影响不显著.高浓度O3和CO2复合处理的叶片O2^·-产生速率、H2O2和MDA含量和电解质外渗率上升不明显,说明高浓度CO2缓解了高浓度O3对蒙古栎叶片的氧化胁迫.复合处理的叶片SOD、CAT、APX活性以及AsA和总酚含量显著高于O3处理的叶片(P〈0.05),说明高浓度CO2缓解了高浓度O3对叶片抗氧化系统的消极影响.
By using open top chamber fumigation method, this paper studied the effects of elevated O3 (≈80 nmol·mol^-1) and CO2 (≈700 μmol·mol^-1), single and in combination, on the active oxygen metabolism of Quercus mongolica leaves. Elevated O3 increased the superoxide anion radical (O2^·-) generation rate, hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents, and electrolyte leakage (EL) (P〈0.05), but decreased the superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) activities and ascorbate (AsA) content (P〈0.05); while elevated CO2 had no significant effects on the active oxygen metabolism of Q. mongolica leaves. The combination of elevated O3 and CO2 had less effects on the O2^·- generation rate, H2O2 and MDA contents, and EL, indicating that elevated CO2 alleviated the oxidative stress of elevated O3 on Q. mongolica leaves, but increased the SOD, APX, and CAT activities and the AsA and total phenol contents, compared with elevated O3 (P〈0.05), indicating that elevated CO2 alleviated the negative effects of elevated O3 on Q. mongolica leaf antioxidant system.