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中文摘要：
目的 探讨碘过量对Fisher大鼠甲状腺细胞（FRTL）线粒体超氧化物生成和膜电位（△ψ）的影响.方法 FRTL细胞分别以10^-4mol/L碘化钾（KI）、10 U/L促甲状腺素（TSH）、10^-4mol/L KI＋10 U/L TSH 处理24 h,利用甲基噻唑基四唑（MTT）比色法检测FRTL细胞增殖情况,利用MitoSOX探针通过活细胞影像法检测线粒体超氧化物生成,利用罗丹明123（rh123）通过荧光分光光度计检测△ψ的变化.结果 细胞增殖情况,KI组（0.794±0.144）明显低于对照组（1.000±0.183,P〈0.05）,TSH组（1.215±0.156）明显高于对照组（P〈0.05）,KI＋TSH组（1.025±0.254）与对照组比较差异无统计学意义（P〉0.05）,但明显高于KI组（P〈0.05）;细胞MitoSOX平均荧光强度（MFI）,KI组和KI＋TSH组（18.16±6.57、13.33±2.92）明显高于对照组（9.74±3.24,P均〈0.05）,TSH组（6.64±2.15）明显低于对照组（P〈0.05）,但KI＋TSH组明显低于KI组（P〈0.05）;细胞rh123 MFI,KI组和KI＋TSH组（210 593±31 328、295 525±34 243）明显低于对照组（407 824±37 198,P均〈0.05）,而KI＋TSH组明显高于KI组（P〈0.05）,TSH组（411 187±72 852）与对照组比较差异无统计学意义（P〉0.05）.结论 碘过量（10^-4mol/L KI）能造成FRTL细胞线粒体过氧化损伤,抑制细胞增殖,10 U/L TSH能够促进FRTL细胞增殖,减轻碘过量对FRTL细胞线粒体的过氧化损伤.
英文摘要：
Objective To investigate the effects of iodine excess on mitochondrial superoxide production and mitoehondrial membrane potential（△ψ）changes in Fisher rat thyroid cell line（FRTL）cells.Methods FRTL cells were treated with 10^-4mol/L potassium iodine（KI）,10 U/L thyrotropin（TSH）,10^-4 mol/L KI＋10 U/L TSH respectively for 24 h.Effects on cell proliferation were assayed by methyl thiazolyl tetrazolium（MTT）colorimetric method.Changes of mitochondrial superoxide production and △ψ were measured by live cell imaging and spectrofluorometer using MitoSOX and rhodamine 123（rh123）respectively.Results Absorbance（A）in the KI group （0.794±0.144）showed a significant decline compared to the control group（1.000 ±0.183,P〈0.05）,whereas a significant elevation was observed in the TSH group（1.215±0.156,P〈0.05）.No significant differences was found between the KI＋TSH group（1.025±0.254）and the control group（P〉0.05）,but the former was marked higher than the KI group（P〈0.05）.Compared to the control group（9.74±3.24）.MitoSOX mean fluorescence intensity （MFI）in the KI and KI＋TSH groups（18.16±6.57,13.33±2.92）were significantly increased（all P〈0.05）,which was a significant decline in the TSH group（6.64±2.15,P〈0.05）.MitoSOX MFI in the KI＋TSH group was lower than the KI group（P〈0.05）.Rh123 MFI in the KI and KI＋TSH groups（210 593±31 328,295 525±34 243）showed significant decline than the control group（407 824±37 198,all P〈0.05）.Compared with the KI group.the KI＋TSH group pronouncedly attenuated the reduction of Rh 123 MFI（P〈0.05）.No significant differences of Rh 123 MFI were found between the TSH group（411 187 ± 72 852） and the control group（P 〉 0.05）. Conclusion Iodine excess （10^-4 mol/L KI） may lead to peroxide damage on the mitochondria of FRTL cells, and cell proliferation is inhibited. Combining treatment with 10 U/L TSH may attenuate mitochondrial peroxide damage and inhibition of cell prolifera
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