中文摘要：

将六氯环三磷腈与分子量为600的超支化寡聚乙烯亚胺在干燥氯仿中反应,合成了可降解的交联型聚合物.利用1HNMR表征了聚合物的结构,并用GPC测试了聚合物的分子量.研究了其作为非病毒基因载体的性能,聚合物载体与DNA形成的复合物颗粒粒径为150nm,Zeta电位为30～40mV,凝胶阻滞电泳显示聚合物/DNA在质量比为0.4时能够将DNA完全阻滞.体外转染实验结果表明,载体对HeLa细胞的最佳转染效率为PEI-25K的3倍;聚合物浓度为20μg/mL时,细胞存活率仍然大于80%,材料的细胞毒性低,生物相容性好,具有良好的生物医学应用前景.

英文摘要：

Hyperbranched oligo（ ethyleneimine） with molecular weight of 600 was carried out reaction with hexachlorotriphosphazene in anhydrous chloroform to synthesize a degradable and cross-linked polymer as a non-viral gene carrier. The 1H NMR spectra confirmed the formation of the polymer and its average molecular weight was obtained by GPC with HAc/NaAc buffering solution of pH = 4. 4 as fluent phase. The polymer compressed DNA into stable complexes in diameter of about 150 nm and zeta potential of 30—40 mV measured by dynamic light scattering. Besides,when the mass ratio of polymer and DNA was 0. 4,the plasmids were fully retarded in 1% agarose gel by gel retardation electrophoresis. The cytotoxicity and transfection efficiency were characterized by HeLa cells in vitro. It was indicated that the best transfection efficiency of polymer with lower cytotoxicity was three times higher than that of PEI-25K. Therefore,the synthesized polymer may have good potential applications as non-viral gene carriers.