中文摘要：

目的：建立测定暴马子皮药材中2个成分（紫丁香苷、sinapylaldehyde 4-O-β-D-glucopyranoside）的高效液相色谱分析方法。方法：采用Phenomenex Luna C18（4.6 mm×250 mm,5μm）色谱柱,流动相为甲醇（B）-0.1%醋酸水溶液（A）,梯度洗脱,流速1.0 mL·min-1,DAD检测器,检测波长：284 nm;柱温25℃。结果：紫丁香苷、sinapylaldehyde 4-O-β-D-gluco-pyranoside的线性范围分别为0.2～0.8μg（r=0.9995）和0.04～0.16μg（r=0.9993）,平均加样回收率（n=6）分别为99.9%（RSD=2.1%）和99.5%（RSD=2.9%）。结论：不同产地暴马子皮药材中所含紫丁香苷、sinapylaldehyde 4-O-β-D-gluco-pyranoside的含量存在显著性差异。本方法操作简便,结果准确,重复性好,可用于中药暴马子皮药材的质量控制。

英文摘要：

Objective：To develop an HPLC method for the determination of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside in Syringae Cortex.Methods：The separation was performed on a Phenomenex Luna C18（4.6 mm×25 0 mm,5 μm） column.The mobile phase was composed of methanol and water solvent containing 0.1% acetic acid with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 284 nm.The column temperature was set at 25 ℃.Results：The calibration curves were linear in the ranges of 0.2-0.8 μg for syringin（r=0.9995） and 0.04-0.16 μg for sinapyl aldehyde 4-O-β-D-glucopyranoside（r=0.9993）.The average recoveries（n=6） of syringin and sinapyl aldehyde 4-O-β-D-glucopyranoside were 99.9%（RSD=2.1%） and 99.5%（RSD=2.9%）,respectively.Conclusion：The yields of the two components in different origins exhibit significant differences.This method is simple,accurate and repeatable,which can be used for the quality control of Syringae Cortex.