利用乙醇沉淀、凝胶过滤等方法从莲藕中分离纯化得到具有抗氧化活性的多糖复合物LB2,经分析,LB2分子量为18.8 kD,由甘露糖、鼠李糖、葡萄糖、半乳糖和木糖这五种单糖组成,其比例为2:8:7:8:1.LB2显示出较强的抑制HIV-1整合酶体外3′切割活性,可作为一种新型HIV-1整合酶抑制剂.
In this study, precipitation and gel filtration were used to purify an antioxidant polysaccharide LB2 from the roots of Nulumbo nuciferra Gaertn. LB2 was a polysaccharide complex with a molecular weight of 18.8 kDa. LB2 was composed of Mannose, Malotriose, glucose, Galactose and xylose in the molar ratio of 2.. 8. 7. 8. 1. LB2 showed strong inhibito- ry activity on the 3P-processing activity of HIV-1 integrase. It would be a novel natural anti- HIV-1 reagent.