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海涂互花米草与水稻远缘杂交种质资源发掘与创新(Ⅳ)
  • ISSN号:0517-6611
  • 期刊名称:安徽农业科学,Journal of Anhui Agri. Sci.
  • 时间:0
  • 页码:22251-22253
  • 语言:中文
  • 分类:S[农业科学]
  • 作者机构:[1]江苏沿江地区农业科学研究所;南通市羊业产业化协会,江苏如皋薛窑226541, [2]郑州大学生物工程系,河南郑州450052
  • 相关基金:2011年国家自然科学基金项目(31072064);2011年江苏省农业科技自主创新资金项目、探索性研究项目配套资助(TCX(11)4133、CX(11)4060).
  • 相关项目:海涂米草与水稻远缘杂交种质资源发掘与创新
作者: 陈启康|
中文摘要:

[目的】发掘互花米草耐盐、水稻高产种质资源,创制海涂粮饲兼用耐盐水稻新种质。【方法】于2009—2011年开展海涂互花米草与水稻远缘杂交研究,采用海涂互花米草与水稻属间远缘杂交育种方法,同时采用四选一突破结合技术一海涂种植筛选、细胞学检测筛选、回交表型筛选、分子标记辅助筛选结合技术,寻找海涂粮饲兼用耐盐水稻新种质。【结果】2009-2010年实际远缘杂交成功率为1.39%。7C14、中香1号2种水稻母本与互花米草(…父本远缘杂交材料进行RAPD分子鉴定,发现RH-1—10K205—7C14xH、RH-2—8K157-7C14xH、RH-13—9H5-中香1号xH具有与互花米草亲本相同的条带,而同时在水稻亲本中缺失的情形,表明以上远缘杂交种具有互花米草亲本遗传成分;水稻7K339母本与互花米草父本远缘杂交材料经RAPD分子鉴定,杂交种RH-5—10K215、RH-6—8K48、RH-12—9H9、RH-14—9H8、RH-16-9H28具有与互花米草亲本相同的条带,而同时在水稻亲本中缺失的情形,表明以上远缘杂交种具有互花米草亲本遗传成分。其余杂交种与互花米草亲本和水稻亲本也存在不同程度的变异。【结论]育出耐盐优质饲料稻新品种,不但解决了沿海开发、盐碱地治理中急需解决的耐盐作物品种问题,而且解决了草食动物精粗饲料问题,同时对于资源利用、农业增效、粮食安全、耕地战略等方面有着重要的科学意义,具有广阔的应用前景。

英文摘要:

[Objective] The aim was to explore salt-resistant Spartina alterniflora and high-yield rice with germplasm resource in order to create new salt-resistant rice species with dual-purpose of food and forage. [Method] From 2009 to 2011, re- searches on distant hybridization of Spartina alterniflora and Oryza Sativa have been conducted with method of distant hybridization breeding. On the other hand, break- through and combination techniques (four selected one) were adopted to find the new rice species, including techniques of planting and selection in seashore, cyto- logical detection and selection, phenotype selection of backcross, and molecular marker assisted selection. [Result] Success rate of distant hybridization from 2009 to 2010 was 1.39%. RAPD molecular identification of 7C14 and rice female parent of Zhongxiang No.l, seed 2 was carried on with distant hybridization of male parent of Spartina altemiflora (H). The results showed that bands same with Spartina alterni- flora parents were found in RH-1-10K205-7C14xH, RH-2-8K157-7C14xH, and RH-13- 9H5-Zhongxaing No.1 xH. Considering absent situation in rice parents, distant hy- bridization strains above were found with same parental genetic element as Spartina alterniflora. Female parent of rice 7K339, however, was under RAPD molecular identification with male parent of Spartina alterniflora. The result showed that bands same with Spartina alterniflora parents were found in RH-5-10K215, RH-6-8K48, RH- 12-9H9, RH-14-9H8 and RH-16-9H28. Considering absent situation in rice parents, distant hybridization strains above were found with same parental genetic element as Spartina altemiflora. Rest hybrid strains were found variance with Spartina alterniflora parents and rice parents in varying degrees. [Conclusion] New and excellent rice species of dual-purpose solves problem not only about salt-resistant species badly needed in coastal development and treatment of saline and alkaline land, but about fine fodder and roughage for herbivore, as well. What's more,

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期刊信息
  • 《安徽农业科学》
  • 北大核心期刊(2008版)
  • 主管单位:安徽省农业科学院
  • 主办单位:安徽省农业科学院
  • 主编:曹淑华
  • 地址:合肥市庐阳区农科南路40号
  • 邮编:230031
  • 邮箱:ahnykx@163.com
  • 电话:0551-65148869 65160973
  • 国际标准刊号:ISSN:0517-6611
  • 国内统一刊号:ISSN:34-1076/S
  • 邮发代号:26-20
  • 获奖情况:
  • 1995年获安徽省优秀期刊三等奖,1998年获安徽省优秀期刊三等奖,2002年获第三届全国优秀农业期刊奖,2005年获第四届全国优秀农业期刊一等奖,2005年获安徽省优秀科技期刊一等奖,2006年获第五届全国农业期刊金犁奖学术类一等奖,第四届华东地区优秀期刊,2009年安徽省优秀期刊,RCCSE中国权威学术期刊(A+),中国农业核心期刊(2010)
  • 国内外数据库收录:
  • 美国化学文摘(网络版),日本日本科学技术振兴机构数据库,中国北大核心期刊(2004版),中国北大核心期刊(2008版)
  • 被引量:163721