中文摘要：

目的探讨旋毛虫成虫排泄分泌蛋白（AES）对盲肠结扎穿孔术（CLP）诱导的小鼠脓毒症的抑制作用。方法将48只BALB/c小鼠随机分为3组,即假手术对照组（PBS＋sham组,A组）、脓毒症组（PBS＋CLP组,B组）、AES蛋白治疗组（AES＋CLP组,C组）。A组和B组于CLP术后经腹腔注射200μl PBS,C组术后经腹腔注射含25μg AES蛋白的等量PBS。每组随机抽取8只,建模后观察各组小鼠的一般情况及96 h生存率;每组其余8只小鼠于建模后12 h取肝、肺、肾,HE染色观察病理改变;以酶联免疫吸附试验（ELISA）检测小鼠血清中TNF-α、IL-1β、IL-6、IL-10及TGF-β细胞因子水平的变化。结果 3组小鼠96 h生存率差异有统计学意义（χ^2=21.16,P〈0.05）;与A组（100%）相比,B组小鼠生存率（0）降低（P〈0.05）,且肺、肝及肾脏损伤明显加重;经AES治疗后,C组存活率（75%）明显增加（P〈0.05）,小鼠肺脏、肝脏及肾脏病理损伤亦显著缓解。3组小鼠血清中的促炎因子TNF-α（F=27.11,P〈0.05）、IL-1β（F=18.75,P〈0.05）及IL-6（F=100.93,P〈0.05）水平差异均有统计学意义;B组小鼠的促炎因子水平均高于A组（P均〈0.05）;经AES治疗后,C组小鼠上述3种促炎因子水平较B组均显著下降（P均〈0.05）。3组小鼠血清中的调节性细胞因子IL-10（F=10.88,P〈0.05）及TGF-β（F=11.37,P〈0.05）水平差异亦有统计学意义;经AES治疗后,C组小鼠IL-10及TGF-β分泌水平较B组亦明显增加（P〈0.05）。结论旋毛虫AES对CLP诱导的BALB/c小鼠脓毒症有显著的缓解作用。

英文摘要：

Objective To observe the effect of excretory/secretory products from Trichinella spiralis adult worms（AES）on cecal ligation and puncture（CLP）-induced sepsis in mice. Methods Forty-eight BALB/c mice were randomly divided into 3groups：a sham operation group（PBS ＋ sham group,Group A）,a CLP-induced sepsis group（PBS＋ CLP group,Group B）and an AES treatment group（AES＋ CLP group,Group C）. The mice of each group were intraperitoneally injected with 25 μg of AES or PBS only as a control in a total volume of 200 μl. Eight mice from each group were selected randomly for survival analysis of 96 hours. The other 8 mice in each group were observed for pathological changes in the lung,liver and kidney tissues by HE staining 12 h after CLP,and then determined for the detection of cytokines including TNF-α,IL-1β,IL-6,IL-10 and TGF-β in the sera by ELISA. Results The difference among the survival rates of mice in the 3 groups was statistically significant（ χ2= 21.16,P 0.05）. Compared to Group A（100%）,the survival rate of mice in Group B（0）decreased significantly（P〈0.05）,and also the pathological damage degrees in the lung,liver and kidney tissues of the mice in Group B increased significantly after CLP. Compared with the mice in group B,the survival rate of those in Group C（70%）increased significantly（P 〈0.05）,and the pathological damage degrees in the lung,liver and kidney tissues of the mice in Group C decreased significantly after the treatment with AES. The differences among the levels of pro-inflammatory cytokines TNF-α（F = 27.11,P〈0.05）,IL-1β（F = 18.75,P 0.05）and IL-6（F = 100.93,P〈0.05）in the sera of the mice in the three groups were statistically significant. Compared with the mice in Group A,the levels of the 3 cytokines of those in Group B increased significantly（all P〈0.05）. However,after the treatment with AES,the levels of the pro-inflammatory cytokines of those in Group C decreased significantly（all P〈 0.05）. The differences amo