用免疫组织化学方法研究脑啡肽(ENK)在极危物种朱鹮(Nipponia nippon)脑内的分布,结合计算机图像分析仪检测免疫阳性细胞和末梢的灰度值.ENK阳性细胞、纤维和终末分布如下:发声核团有原纹状体中间区腹部、丘脑背内侧核外侧部、中脑丘间核、中脑背内侧核、延髓舌下神经核.听觉中枢有丘脑卵圆核壳区、中脑背外侧核壳区、脑桥外侧丘系腹核、上橄榄核、耳蜗核等.内分泌核团有视前区前核、旧纹状体增加部、下丘脑外侧核、下丘脑腹内侧核等.结果表明,朱鹮脑内ENK可能对发声、听觉和下丘脑内分泌的生理活动有一定的调制作用.
The distribution of enkephalin in the brain of a male Nipponia nippon was investigated by using the immunohistochemieal method, and the numerical value of gray density was measured by the image processing system in computer. The enkephalin labeled terminals and cells distributed in the vocal control nuclei such as archistriatum intermedium pars ventralis (AiV), dorsolateral nucleus of the anterior thalamus (DLM), the nucleus iutercollieularis (ICo), nucleus domalis medialis (DM), pars tracheosyringealis (nXIIts), and nucleus hypoglossin ( nXII ) of N. nippon. In the auditory center such as the nucleus ovoidalis shell (Or shell), the shell regions of mesencephalicus lateralis, pars dorsalis (Mid shell), nucleus ventralis lemnisci lateralis(VLV), superior olivary nucleus(SO) and the cochlear nuelei(nCo) ete; in the endocrine nuclei such as nucleus preoptieus anterior (POA), paleostriatum primitivum (PA), nucleus ventromedialis hypothalamie (VMN), and nucleus lateralis hypothalamie (LHy) etc. The results indicate that enkephalin may play an important role in the vocal, auditory and endocrine function of hypothalamus.