为了对重离子辐照致DNA损伤中的自由基作用进行评估,本文利用γ射线对添加了各种自由基清除剂(甘露醇,维生素C和茶多酚)的质粒DNA进行辐照,凝胶电泳分析的结果表明,三种自由基清除剂对DNA均有很好的保护作用.在相同自由基清除剂浓度的情况下,甘露醇比维生素C具有更强的清除自由基的作用.与重离子辐照情况相比,这几种自由基清除剂对γ射线产生的自由基的清除作用更为明显.
To evaluate the effect of free radicals on DNA damage induced by heavy ions, pUC19 plasmid DNA solution with different kinds of scavengers (Mannitol, Vitamin C and Tea Polyphenols) is irradiated by γ rays. The DNA damages are analysed by gel electrophoresis and Alpha Innotech digital imaging system. The results show that all of the three kinds of scavengers can significantly protect DNA; mannitol is more effective than Vitamin C under the same condition; and their capacity of scavenging free radicals induced by γrays is more remarkable than those induced by heavy ions.