中文摘要：

采用DNA和蛋白质吸收带的Kubelka-Munk光谱函数对人结肠腺癌进行了鉴别诊断,实验采用带积分球附件的分光光度计获取组织的漫反射光谱。结果表明：在250～650 nm,结肠上皮组织的癌变导致其上皮组织在DNA吸收带的Kubelka-Munk光谱函数f（r∞）及其对数log[f（r∞）]的平均值在260 nm处都有非常显著性的差异,其差异分别为218%（p〈0.05）和68.5%（p〈0.05）。结肠上皮组织的癌变导致其上皮组织在蛋白质吸收带的Kubelka-Munk光谱函数f（r∞）及其对数log[f（r∞）]的平均值在280 nm处也都有非常显著性的差异,其差异分别为208%（p〈0.05）和59.0%（p〈0.05）。结肠上皮组织的癌变导致其上皮组织在β-胡罗卜素吸收带（480 nm处）的Kubelka-Munk光谱函数f（r∞）及其对数log[f（r∞）]的平均值在480 nm处也都有非常显著性的差异,其差异分别为41.7%（p〈0.05）和32.9%（p〈0.05）。可见,结肠上皮组织的癌变导致其上皮组织中的DNA、蛋白质和β-胡罗卜素的含量都发生了非常显著的变化。这结论为快速、低成本、非入侵的结肠腺癌的光活检提供一些有益的参考。

英文摘要：

Differential diagnosis of human colon adenoma was studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 590 to 1 064 nm pathological changes of colon epithelial tissues were induced so that there were Significant differences in the averaged values of the Kubelka-Munk function f（r∞ ） and logarithmic Kubelka-Munk function log[f（r∞）] of the DNA absorption bands at 260 nm between normal and adenomatous colon epithelial tissues, and the differences were 218% （p〈0.05） and 68. 5% （p〈0. 05） respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f（r∞ ） and logarithmic Kubelka-Munk function log[f（r∞ ）] of the protein absorption bands at 280 nm between normal and adenomatous colon epithelial tissues, and the differences were 208% （p〈0. 05） and 59.0 % （p〈 0. 05） respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f（r∞ ） and logarithmic Kubelka-Munk function log[f（r∞ ）] of the β-carotene absorption bands at 480 nm between normal and adenomatous colon epithelial tissues, and the differences were 41.7% （p〈0.05） and 32. 9% （p〈0.05） respectively. Obviously, pathological changes of colon epithelial tissues were induced so that there were significant changes in the contents of the DNA, protein and β-carotene of colon epithelial tissues. The conclusion can be applied to rapid, low-cost and noninvasive optical biopsy of colon adenoma, and provides a useful reference.