中文摘要：

原型泡沫病毒（prototypic foamy virus,PFV）属于反转录病毒科泡沫病毒亚科.为了在细胞水平定量检测PFV的感染,建立了以萤火虫荧光素酶为报告基因的指示细胞系.在幼小仓鼠肾细胞BHK-21中转染含有PFV LTR启动子及其控制下的荧光素酶报告基因的质粒,经过G418筛选得到稳定整合的细胞系.当PFV感染指示细胞系时,其反式激活因子Tas激活LTR启动子,诱导下游报告基因的表达,通过检测荧光素酶的活性即可对PFV的感染进行定量检测.结果表明,该细胞系可特异指示PFV的感染,相比于传统观察细胞病变的方法,该指示细胞系更为灵敏,是PFV相关研究的有力工具,具有潜在的应用价值.

英文摘要：

Prototypic foamy virus（PFV）belongs to the Spumaretrovirinae subfamily of the Retroviridae family.In order to quantitate PFV infection in vitro,a PFV indicator cell line （PFVL）was established.Baby hamster kidney cells were transfected with reporter plasmid which contains the firefly luciferase gene driven by a PFV long terminal repeat promoter.The PFV transactivator Tas could activate promoter activity of LTR to express luciferase after PFV infection.Then the infection could be quantified by detection of luciferase activity.PFV indicator cell line could detect PFV infection specifically.Compared with standard assays used to detect PFV infection,the PFVL-based assay is more sensitive than the CPE-based assay.In brief,the PFV indicator cell line is an easy,robust and quantitive method for monitoring PFV infection.