中文摘要：

利用2-DE差异显示和质谱分析研究了龙眼（Dimocarpus longan Lour.）成花逆转3个不同时期花芽的蛋白质组变化。在花穗叶片尚未展开期（Ⅰ期）共检测到1012个蛋白点,花穗叶片展开但未转绿期（Ⅱ期）1034个蛋白点,花穗叶片转绿期（Ⅲ期）1098个蛋白点。发现19个差异表达的蛋白质,其中15个上调表达,4个下调表达。经MALDI-TOF-TOF/MS质谱分析和蛋白质数据库检索,有11个差异表达的蛋白质得到鉴定,分别为异黄酮还原酶相似蛋白（No.1）、二硫键异构酶前体相似蛋白（No.2）、拟南芥同源的某未知蛋白（No.3）、脱落胁迫成熟相似蛋白（No.4）、ATP合酶β亚基（No.6）、真核翻译起始因子（No.9）、DNA结合蛋白MNB1B（No.10）、胞质磷酸甘油酸激酶（No.14）、过氧化物酶（No.16/17）和晚期胚胎丰富相似蛋白（No.19）。这些蛋白分别在能量代谢、转录翻译、物质运输、信号转导以及胁迫生理等方面与龙眼成花逆转密切相关。

英文摘要：

Proteomic approaches were used to analyze the changes of flower bud proteomes in different floral reversion periods in longan （Dimocarpus longan Lour. ）. Nearly 1 012 proteins were reproducibly detected in the period of floral leaf without open out （stage Ⅰ）, 1 034 proteins were reproducibly detected in the period of fioral leaf open out but not turning to green （stage Ⅱ ）, 1 098 proteins were reproducibly detected in the period of floral leaf turning to green （stage Ⅲ）. Nineteen proteins differentially expressed were detected on 2 DE gels. Among these proteins, fifteen were up-regulated and 4 were down-regulated. Eleven differential proteins were identified by both MALDI-TOF-TOF/MS and protein database searching. They were isoflavone reductase-like protein （No. 1）, disulfide-isomerase precursor-like protein（No.2）, unknown protein ofArabidopsis thaliana（No.3）, abscisic stress ripening-like protein（No. 4）, putative ATP synthase beta subunit（No.6）, eukaryotic translation initiation factor 5A-2（elF-5A-2） （elF-4D） （No.9）, DNA-binding protein MNB 1B （No. 10）, cytosolic phosphoglycerate kinase （No. 14）, peroxidase （No. 16 / No. 17）, and late embryogenesis-like protein （No. 19）. All of these differential proteins are closely related to floral reversion of longan in energy metabolism, transcription and translation, substance transportation and signal transduction.