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中文摘要：
将生物素共价键合到微悬臂的硅表面,利用微悬臂传感技术在流动注样条件下研究了生物素-亲和素的动态作用过程,同时利用原子力显微镜（AFM）力刻蚀技术验证了生物素化硅表面结合亲和素的有效性.结果显示亲和素与生物素作用产生较大的表面应力驱动微悬臂偏转,悬臂偏转的程度和速率与亲和素的浓度相关,亲和素浓度越大,微悬臂偏转程度也越大,同时反应速率也更快.这项工作为开发新型的无标记检测技术,研究生物分子之间相互作用提供了有益的探索.
英文摘要：
The dynamic interaction process of biotin-avidin was investigated by biotin covalently modified microcantilever sensor in real-time in a flowing cell.The specific interaction was confirmed by the evidence that the avidin layer can be mechanically removed with atomic force microscopy nanolithography.The de-flection of the modified cantilever increased with concentration of avidin.Microcantilever biosensor pro-vides a good method to explore novel label-free technology and monitor various interactions between bio-molecules.
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