中文摘要：

将枯草芽孢杆菌、白腐真菌和分离筛选菌群分别用于芘和苯并[a]芘污染土壤的修复,比较其修复性能,并对表面活性物剂Tween80和葡萄糖对修复效果的影响规律做了初步探讨。结果表明,60 d时,枯草芽孢杆菌和分离筛选菌修复芘污染的土壤,芘的残留率分别为7.58%±0.59%和8.69%±1.35%,空白残留率为48.18%±0.56%;对于枯草芽孢杆菌、白腐真菌和分离筛选菌对土壤中苯并[a]芘的修复,在60 d时,苯并[a]芘的残留率分别为66.27%±3.03%,62.28%±2.54%和67.67%±2.98%,空白残留率为70.25%±1.62%。60 d时,加入Tween80后芘的残留率比仅加入枯草芽孢杆菌的降低了2.41%,而苯并[a]芘,残留率比仅加入枯草芽孢杆菌和白腐真菌的分别降低了12.72%和14.59%;加入葡萄糖后,芘的残留率比仅加入枯草芽孢杆菌降低了2.30%,而苯并[a]芘,60 d残留率比仅加入枯草芽孢杆菌和白腐真菌的分别降低了5.39%和11.93%。

英文摘要：

Degradation of pyrene and benzo[a]pyrene in soils by Bacillus subtilis,white rot fungus and mixed degrading bacteria that were isolated from oil contaminated soil were investigated.Effects of Tween80 and glucose on biodegradation of PAHs were also preliminarily discussed.Results showed that the residual rate for pyrene was 7.58%±0.59% and 8.69%±1.35% on the 60th day when degraded by Bacillus subtilis and the isolated bacteria respectively,while it was 48.18%±0.56% for the uninoculated control soil.Benzo[a] pyrene also showed residual rate with 66.27%±3.03%,62.28%±2.54% and 67.67%±2.98% on the 60th day in remediation soils by Bacillus subtilis,white rot fungus and the isolated degrading bacteria respectively.And in the control soil,70.25%±1.62% of the initial benzo[a]pyrene was left after 60d.After adding Tween80,the residual rate of pyrene by Bacillus subtilis decreased by 2.41%.As for benzo[a]pyrene,the residual rate decreased by 12.72% for Bacillus subtilis and 14.59% for white rot fungus.Results showed that the residual rate of pyrene by Bacillus subtilis decreased by 2.30% after adding glucose,while it decreased by 5.39% and 11.93% for benzo[a]pyrene degraded by Bacillus subtilis and white rot fungus respectively.