中文摘要：

合成生物学诺言将由装配整个小径的分开的模块简化新陈代谢的小径的构造。这为象 polyhydroxyalkanoates (公众房产管理局) 那样的工业产品的微生物引起的生产给新途径。在这研究，(3-hydroxybutyrate-co-3-hydroxyhexanoate )(PHBHHx ) 由 Pseudomonas stutzeri， 1317 从无关的碳采购原料 poly 生产例如葡萄糖， P 的 phaC1-phaZ-phaC2 操纵子。stutzeri 1317 被击倒产生 PHA 缺乏的变异的 P。stutzeri 1317LF。然后，包含 phaCAhAReBRe， phaCAhBReGPp 和 phaCAhPAh 的三个模块被介绍进 P。stutzeri 1317LF 独立。饮料烧瓶结果显示先锋供应和公众房产管理局 synthase 活动是为 P 的 PHBHHx 累积的重要因素。stutzeri 1317LF。而且，从不同的碳资源的 recombinants 的 PHBHHx 累积被执行。最高的 PHBHHx 内容是 23.7%( 由质量) 与 58.6%( 由鼹鼠) 3HB 部分。这些结果为进一步改进 P 的 PHBHHx 累积提供基础。从无关的碳来源的 stutzeri。

英文摘要：

Synthetic biology promises to simplify the construction of metabolic pathways by assembling the de- tached modules of the whole pathway. This gives new approaches for the microbial production of industrial products such as polyhydroxyalkanoates （PHA）. In this study, to produce poly（3-hydroxybutyrate-co-3-hydroxyhexanoate） （PHBHHx） by Pseudomonas stutzeri 1317 from unrelated carbon sources such as glucose, the phaCl-phaZ-phaC2 operon of P. stutzeri 1317 was knocked out to generate the PHA deficient mutant P. stutzeri 1317LF. Then three modules containing phaCahAReBRe, phaCahBReGep and phaCAhPah were introduced into P. stutzeri 1317LF separately The shake flask results indicated that the precursor supply and PHA synthase activity were the vital factors for the PHBHHx accumulation of P. stutzeri 1317LF. Furthermore, the PHBHHx accumulation of the recombinants from different carbon resources were performed. The highest PHBHHx content was 23.7% （by mass） with 58.6% （by mole） 3HB fraction. These results provide basis for further improving the PHBHHx accumulation of P. stutzeri from unrelated carbon sources.