中文摘要：

建立了一套预放大式数字全息显微成像系统,通过对样品进行显微放大,实现了高分辨率的定量相位成像;并通过计算机控制相机自动曝光记录序列的数字全息图实现了动态相位成像。用标准样品验证了系统测量的准确性;以活体洋葱表皮细胞和血红细胞为样品,获得了清晰的定量相位像;以置于水环境的草履虫为样品,实现了动态成像。实验结果表明建立的系统可以实现高分辨率的动态定量相位成像,可以应用于生物活体样品的显微研究。

英文摘要：

Digital holographic microscopy apparatus with pre-magnification has been set up,which can well acquire the quantitative phase images for living biological specimens.In addition,a series of digital holograms can be automatically recorded and movies of holographic phase images of living biological specimens can be created.The accuracy of quantitative imaging is verified by a self-produced stepped transparent sample with known height and refraction.Onion epidermal cells and red blood cells are measured and quantitative high-quality phase images are obtained.The movies of phase images for paramecium are achieved.Experimental results demonstrate that the established system can achieve real-time quantitative high-resolution holographic phase image and can be effectively applied to living biological specimens phase imaging.