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中文摘要：
为探明硫氧还蛋白还原酶（TrxR）在微生物中的保守性,利用宏基因组技术从农药厂废水淤泥中提取环境DNA,并以其为模板直接采用PCR方法进行TrxR基因克隆,最终获得了硫氧还蛋白还原酶基因的部分序列片段。该片段全长476 bp,通过多序列比对及进化树分析,发现其与海杆菌、假单胞菌硫氧还蛋白还原酶基因序列的同源性高达80%以上,氨基酸序列同源性达98%以上,在进化中具有高度的保守性。
英文摘要：
To prove whether thioredoxin reductase（TrxR） is conservative in the microorganisms, metagenomic technique was used for extraction of the soil metagenomic DNA and cloning of TrxR gene from soil metagenomic DNA by PCR technique in this study.Partial TrxR gene with 476 bp was obteined and analysed by multiple sequence comparison and phylogenetic tree analysis.The results showed that it was more than 80%in homology with TrxR genes from Marine bacterium and Pseudomonas,and more than 98%in amio acid homologous alignments.This shows that TrxR has a high degree of conservativeness in the evolution of microorganisms.
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