中文摘要：

利用细胞培养、免疫组化、原位杂交、图像分析技术研究钙信号与缺氧诱发心肌细胞癌基因c-fos、c-myc表达的关系,进一步阐明钙信号转导与以右心肥大衰竭为特征的肉鸡腹水综合征(AS)的关系。结果显示,钙通道拮抗剂异搏安(V)、硝苯吡啶(N)、环孢霉素A(C)显著抑制缺氧引起的癌基因c-fos和c-myc mRNA的表达(c-fosmR-NA:常氧149.6±22.4;缺氧205.4±57.6,缺氧+V151.3±20.1,缺氧+N154.5±25.2,缺氧+C 166.5±22.1;c-myc mRNA:常氧155.2±25.7;缺氧197.4±26.2,缺氧+V 160.5±19.4,缺氧+N 171.3±25.4,缺氧+C158.9±23.6)。同时显著抑制缺氧引起的癌基因c-fos和c-myc蛋白的表达(c-fos蛋白:常氧159.1±37.1,缺氧240.3±49.6,缺氧+V 165.2±52.2,缺氧+N 166.5±49.8,缺氧+C 190.8±33.6;c-myc蛋白:常氧149.6±24.9,缺氧236.3±55.4,缺氧+V178.9±42.1,缺氧+N174.4±36.3,缺氧+C 16...

英文摘要：

The purpose of this experiment was to explore the relationship between calcium signal transduction and expression of c-fos and c-myc in broiler s cardiac muscle cell(CMC) induced by hypoxia.Hypoxia is the main inducement of ascites syndrome(AS).CMC hypertrophy were the important cause of AS.The effect of calcium signal transduction on the oncogene c-fos and c-myc expression in CMC induced by hypoxia was observed by immunohistochemistry,in situ hybridization and image analysis methods.The results showed that...