中文摘要：

通过盆栽试验,研究了在低氮（不施氮）和高氮（施氮0.2 g·kg^-1）水平下接种不同种类丛枝菌根（AM）真菌[Funneliformis mosseae（BGC-NM03D）、Claroideoglomus etunicatum（BGC-NM01B）和Rhizophagus intraradices（BJ09）]对小麦生长、氮吸收及根内4个硝态氮转运蛋白（NRT）基因、1个辅助蛋白（NAR）基因和2个铵态氮转运蛋白（AMT）基因表达的影响。结果表明,3种AM真菌均能够侵染小麦根系,以R.intraradices菌根的侵染率最高;接种R.intraradices或C.etunicatum能够显著提高小麦的生物量或地上部氮吸收量;无论是高氮还是低氮处理,接种AM真菌后均显著下调了小麦根内NRT、NAR和AMT基因的表达水平,且不同AM真菌调控小麦根内氮转运蛋白基因表达的能力具有明显差异。

英文摘要：

A pot experiment was carried out to investigate the effects of inoculation of different arbuscular mycorrhizal（ AM）fungi [Funneliformis mosseae（ BGC-NM03D）, Claroideoglomus etunicatum（ BGC-NM01B）, Rhizophagus intraradices（ BJ09） ] on growth,nitrogen uptake and expression of 5 nitrate transporter（ NRT NAR） and 2 ammonium transporter（ AMT） genes in roots of wheat under low N（ no N application） and high N（ N 0. 2 g·kg^-1soil） treatments. The results indicated that wheat roots were colonized by all the three AM fungi,and the fungus R. intraradices had the highest colonization rate in both N treatments. The inoculation of R. intraradices and C. etunicatum significantly increased shoot biomass or shoot N accumulation compared with the non-mycorrhizal control. The transcription levels of all the NRT and AMT genes in roots of wheat were down-regulated by inoculating the AM fungi in both N treatments,and different AM fungi exhibited different abilities to regulated the expression of the NRT,NAR and AMT genes.