中文摘要：

目的观察转化生长因子β1（TGF-β1）基因转染后对大鼠树突状细胞（DC）生物学特性的影响。方法将TGF-β1基因导入大鼠DC,G418筛选,检测转染细胞TGF-β1的表达及其生物学活性,并进行混合淋巴细胞反应（MLR）。结果TGF-β1基因转染的DC可以分泌TGF-β1,而且可特异性抑制血管内皮细胞,对T淋巴细胞的增殖有抑制作用。结论利用构建的TGF-β1表达质粒转染大鼠DC,转入的TGF-β1基因可以在DC内稳定表达,并且使DC的生物学特性发生相应的改变。

英文摘要：

[ Objective ] To investigate the effects of transforming growth factor β1 （TGF - β1 ）gene transfer on the biological characteristics of dendritic cell （DC） in rat. [ Methods] DCs were transfected with pAd - TGF - β1 plasmid through lipofectamine mediated gene transfer, and the transfected cells were selected by G418. The biological characteristics of transfected DCs were detected by Western - Blot, vascular endothelial cell growth - inhibition test and mixed lymphocyte reaction （MLR） . [ Results] The transfected cells could excrete TGF - β1 ,and the supernatant could inhibit the growth of mink lung epithelium cell and the proliferation of T lymphocyte cells. [ Conclusions ] DCs transfected by pAd - TGF - β1 can express TGF - β1 stably, and the biological characteristics of transfected DCs are changed corespondingly.