以枸杞叶为原料,采用单因素和正交试验设计研究枸杞叶中黄酮的超声法提取工艺,并研究提取液抑菌活性。结果表明:优化后的提取工艺条件是乙醇浓度(A)80%(体积分数)、料液比(B)1∶30(g/mL)、超声提取时间(C)35 min和超声功率(D)270 W,此时枸杞叶黄酮提取率为5.62%,各因素对黄酮提取率的影响大小顺序依次为A〉C〉B〉D;枸杞叶黄酮提取液对黑曲霉、面包酵母无抑制作用,但其能抑制大肠杆菌(G~-)、金黄色葡萄球菌(G~+)及青霉的生长,最小抑菌浓度分别为10.3、20.6、41.2 mg/g。
The ultrasonic extraction technology of flavonoids from boxthorn leaves with ethanol as extraction solvent was studied by single factor and orthogonal experiments, and the antimicrobial activity of flavonoids ex-tract was also studied.Results showed that optimized extraction technological condition was ethanol concentra-tion(A) 80%(volume ratio), the ratio of solid to liquid(B) 1:30(g/mL), extraction time(C) 35 min and ul-trasonic power(D) 270 W, that the flavonoids extraction rate from boxthorn leaves was 5.62%under the condi-tion, and that various factors influenced the extraction rate in the order A>C>B>D,and that the crude extracts had no effect on the growth of Niger and Saccharomyces cerevisiae, yet the extracts inhibited the growth of E. coli,Staphyloccocus aureus and Penicillium with the MIC was 10.3,20.6,41.2 mg/g respectively.