中文摘要：

将羊种布鲁菌外膜蛋白25（OMP25）基因插入到山羊痘病毒转移载体PGM-TK-I1L-GPT1启动子I1L的下游,构建重组山羊痘病毒转移载体。用脂质体转染法将重组转移载体与山羊痘病毒疫苗株G14-STV44-55共转染绵羊成纤维细胞,在细胞内同源重组。通过霉酚酸药物筛选、纯化,经PCR鉴定获得了重组山羊痘病毒。

英文摘要：

To construct the Recombinant goatpoxvirus expressing keywrod＇s ontside membrance protein （OMP25） gene of B. melitensis, the OMP25 gene was inserted into the downstream of promotor IlL of goatpoxvirus（GPV）transfer vector PGM-TK-IIL-GPT1. Then the recombinant transfer vector was transfected into sheep fibroblast ceils infected with GPV vaccine strain G14-STV44-55 by lipofectamine. The transfected sheep fibroblast ceils were conducted by mycophenolic acid（MPA）to screen and purify recombinant goatpoxvirus（RGPV）. Recombinant goatpoxvirus was successfully obtained and identified by PCR.