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Mining cotton fiber strength candidate genes based on transcriptome mapping
  • ISSN号:1001-6538
  • 期刊名称:科学通报(英文版)
  • 时间:0
  • 页码:4651-4657
  • 语言:中文
  • 分类:S562.032[农业科学—作物学] Q756[生物学—分子生物学]
  • 作者机构:[1]Key Laboratory of Crop Germplasm Resources of Hebei Province, Agricultural University of Hebei, Baoding 071001, China, [2]College of Life Science, Yangtze University, Jingzhou 434025, China
  • 相关基金:Supported by the National Basic Research Program of China (Grant No. 2010CB 126000), National High Technology Research and Development Program of China (Grant No. 2006011001044), and the National Natural Science Foundation of China (Grant Nos. 30671322 and 30871561)
  • 相关项目:棉纤维次生壁加厚期转录组图谱构建及纤维品质QTLs精细定位
作者: 马峙英|
中文摘要:

棉花纤维力量主要在变厚的第二等的房间墙(SCW ) 期间被决定舞台。在 SCW 变厚舞台的 24 25 天柱子开花期(DPA ) ,放大 cDNA 的碎片长度多型性(AFLP ) 被执行构造纤维 transcriptome 组。把组基于这些,棉花纤维力量候选人基因被印射的合成间隔(计算机输入缩微胶卷) 通过扫描的量的特点地点(QTL ) 检测。印射的人口是 Gossypium hirsutum × G 的种间的回交 BC 1 。barbadense。115 家 BC 1 工厂与 102 合格缺席 / 存在被用于组建设从 G 的多态的导出抄本的碎片(TDF ) 。barbadense,和 78 TDF 被分配进给了 462.63 centimorgans (厘米) 的全部的长度的八个 transcriptome 组。二重要 QTL, FS1 和 FS2,分别地被检测并且解释 16.08% 纤维力量和 15.87% 变化。与 FS1 和 FS2 共同分离的六 TDF,除了编码未知蛋白质的,五分别地指向了通常认为的 phosphatidylinositol kinase, trehalose-6-phosphate synthase, MADS 抄写因素,纤维素象 synthase 一样蛋白质和本氨基丙酸氨 lyase。这些功能的基因涉及植物房间墙形态发生或纤维素合成新陈代谢过程,并且被看作控制棉花纤维力量的候选人基因。

英文摘要:

Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping (CIM) through quantitative trait locus (QTL) scanning. The mapping population was the interspecific backcross BC1 of Gossypium hirsutum × G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments (TDFs) from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans (cM). Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6 phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogenesis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength.

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