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华南野生大豆GmPAP1基因多样性分析
  • 期刊名称:大豆科学,2010,29(6)
  • 时间:0
  • 分类:S565.1[农业科学—作物学]
  • 作者机构:[1]华南农业大学农学院,广东广州510642, [2]江西省农业科学院作物研究所,江西南昌330200
  • 相关基金:国家自然科学基金资助项目(30771364)
  • 相关项目:我国南亚热带地区野生大豆资源的遗传多样性及耐酸铝低磷能力的鉴定研究
中文摘要:

GmPAP1属低磷诱导基因,通过同源克隆测序,分析68份来源于华南亚热带地区江西、湖南、福建、广西等地野生大豆GmPAP1基因的多样性。与原基因序列GmPAP1(AF236108)比较,68份种质资源共有22个SNPs和6个Indels,22个SNPs中有13处发生转换,9处发生颠换,转换所占比例接近2/3,有14个发生了非同义突变,表明该基因在不同地区的大豆中具有较高的多样性。

英文摘要:

GmPAP1 is a gene induced by low-phosphorus condition,the sequence polymorphisms of GmPAP1 in comparison with the control gene sequence(AF236108)from Genebank were analyzed by cloning in 68 wild soybean genotypes mainly from the subtropical zone: Jiangxi,Hunan,Fujian province and Guangxi Autonomous Region in South China.The results showed that,22 SNPs and 6 Indels were found.Among these polymorphic sites,13 transition,9 transversion and 14 non-synonymous mutations were detected,indicating the abundance of gene diversity among these genotypes.

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