GmPAP1属低磷诱导基因,通过同源克隆测序,分析68份来源于华南亚热带地区江西、湖南、福建、广西等地野生大豆GmPAP1基因的多样性。与原基因序列GmPAP1(AF236108)比较,68份种质资源共有22个SNPs和6个Indels,22个SNPs中有13处发生转换,9处发生颠换,转换所占比例接近2/3,有14个发生了非同义突变,表明该基因在不同地区的大豆中具有较高的多样性。
GmPAP1 is a gene induced by low-phosphorus condition,the sequence polymorphisms of GmPAP1 in comparison with the control gene sequence(AF236108)from Genebank were analyzed by cloning in 68 wild soybean genotypes mainly from the subtropical zone: Jiangxi,Hunan,Fujian province and Guangxi Autonomous Region in South China.The results showed that,22 SNPs and 6 Indels were found.Among these polymorphic sites,13 transition,9 transversion and 14 non-synonymous mutations were detected,indicating the abundance of gene diversity among these genotypes.