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中文摘要：
以甘薯蛋白为原料,采用复合菌共生发酵法制备生物活性肽。通过Sephadex G-25型色谱柱对发酵产物进行分离,并利用高效液相色谱法做进一步的分析验证,测定了产物的DPPH自由基清除能力及还原能力。结果表明,甘薯蛋白在枯草芽孢杆菌与黑曲霉比例1.5∶1.0,以10%的接种量在5%甘薯蛋白发酵培养基中发酵48 h后,可水解为具有一定DPPH自由基清除能力以及还原能力的小分子肽类物质。
英文摘要：
Using sweet potato protein as raw material,the biological activity peptide is prepared by the method of compound bacteria symbiotic fermentation. The fermentation products are separated by Sephadex G- 25 column, and further analysis is made by using high performance liquid chromatography,the free radical scavenging ability and reducing ability of DPPH· are determined. The results show that Bacillus subtilis and Aspergillus niger,according to the proportion of 1.5∶1.0,with 10%of the inoculum amount,in the fermentation medium of 5% sweet potato protein,after 48 h fermentation,sweet potato protein can be hydrolyzed to small molecular peptides with DPPH·radical scavenging ability and reducing power.
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