茶树(Camellia sinensis(L.)O.Kuntze)中含有丰富的多酚类化合物,统称为荼多酚,包括黄烷醇、黄酮、黄酮醇、花青素、原花青素和酚酸类等。对茶树根中原花青素的提取工艺进行优化,筛选出最佳的提取工艺条件:丙酮浓度80%、提取时间35min、提取温度50℃、加酸量0.25%。对多酚的检测方法-香草醛法、对二甲氨基肉桂醛(DMACA)法进行了系统性的优化,结果表明香草醛法最优条件:0~25℃、反应时间15min、硫酸体积分数为50%、香草醛浓度为10g·L^-1。对二甲氨基肉桂醛(DMAcA)法最优条件:0℃、5min内、盐酸浓度1.2mol·L^-1、DMACA浓度2g·L^-1。
There are abundant polyphenol compounds in tea, collectively referred to as tea polyphenols, including flavanol, flavonoids, flavonols, anthocyanosides, proanthocyanidins and phenolic acids, etc. In this experiment, we systematically optimized those parameters affecting proanthocyanidins extraction rate. The best extraction conditions were as follows: 80% acetone, extraction for 35 min at 50℃ with 0.25 %acid additives. The determination conditions with Vanillin method and Dimethylaminocinnamaldhyde method were also systemati- cally optimized, and the results showed that the optimal condition with Vanillin method was 0-25℃, reaction for 15 min, 50% H2SO4 and 10 g·L^-1 vanillin; while the optimal condition with DMACA method was 0℃, reaction for 5 rain, 1.2 mol·L^-1 HC1, and 2 g·L^-1 DMACA.